The Relationship Of Serum SFAS And VEGF Expression In Chronic Obstructive Pulmonary Disease Patients

Background:Chronic obstructive pulmonary disease(COPD) is characterized by airfiow obstructionthat is not fully reversible.The pathogenesis is not clear,apoptosis mechanism become a hotresearch in the pathogenesis of chronic obstructive pulmonary disease in recent years.ObjectiveTo discuss the the importance of apoptosis in pathogenesis of COPDand found theapoptosis markers which can be used to evaluate COPD disease control level, thus it can beimpossible to found a new and effective treatment for COPD patients by regulatingapoptosis.MethodsThe patients were divided into three groups, Ⅰ group (normal control group) whichcontain20healthy volunteers, Ⅱ group which contain80acute exacerbation phase COPDpatients who were divided into A degree, B degree, C degree and D degree by severity level,respectively, Ⅲgroup which contain80stable phase COPD patients who treated after acuteexacerbation phase.Determined each groups of serum Soluble Fas Ligand (sFas),VEGFlevels and lung function, sFas and VEGF was determined by enzyme-linkedimmunosorbent assay(ELISA)． Results1.The concentration of sFas in acute exacerbation phase COPD patients is(20.12±2.14ng/ml) higher than stable phase（17.90±2.07ng/ml）, the results are significantdifferences (P<0.05), and it is also higher than healthy volunteers（15.15±2.87ng/ml）andthe results are also significant differences (P<0.05). The concentration of sFas in stablephase COPD patients is （17.90±2.07ng/ml） higher than healthy volunteers（15.15±2.87ng/ml)，the results are significant differences (P<0.05).2.The concentration of VEGF in acute exacerbation phase COPD patientsis(510.7±83.12pg/ml) higher than stable phase （416.0±65.78pg/ml）, the results aresignificant differences (P<0.05), and it is lower than healthy volunteers（521.6±35.75pg/m)but the results are no significant differences (P>0.05). The concentration of VEGF in stablephase COPD patients is（416.0±65.78pg/ml）lower than healthy volunteers, the results aresignificant differences (P<0.05).3.The concentration of sFas in all degrees of acute exacerbation phase COPD patientsare difference, but there are no differences between A and B degree (P>0.05) and the resultsare significant differences (P<0.05) between C and D degree.4.The concentration of VEGF in all degrees of acute exacerbation phase COPDpatients are difference, but there are no differences between A and B degree (P>0.05) andthe results are significant differences (P<0.05) between C and D degree.5.There is no correlation between concentration of sFas and FEVl％in COPD patients(P>0.05).6.There is no correlation between concentration of VEGF and FEVl％in COPDpatients (P>0.05).Conclusion:This experiment shows that there is abnormal apoptosis in COPD patients, especiallyin acute exacerbation patients,the concentration of sFas and VEGF closely associated withthe severity of the COPD,so both can be used as indicators to reflect the severity andactivity condition of COPD.