The Expression Levels And The Clinical Significance Of MMP-9and TIMP-1in Post-infectious Obliterans Bronchiolitis Of Children

Objective To investigate extracellular matrix metalloproteinase-9(MMP-9) and thetissue inhibitor of metalloproteinase-1(TIMP-1) expression levels in thebronchoalveolar lavage fluid and blood to explore the clinical significance of theMMP-9and TIMP-1in post-infectious obliterans bronchiolitis of childrenMethod thirteen Children with post-infectious bronchiolitis obliterans was enrolledin the study,ten chilidren with bronchial foreign body（Control group of lavage fluid）and thirteen Children healthy children（Control group of blood）was study as control，serum and bronchoalveolar lavage fluids of Matrix metalloproteinases (MMP)-9、tissue inllibitor of metalloproteines (TIMP)-1was measured by Enzyme linkedimmunosorbent assay(ELISA).Results compared with control group (131.33±43.93),MMP-9of the bronchoalveolarlavage fluids in bronchiolitis obliterans group (2732.81±599.57) were Significantlyincreased(p<0.01), compared with control group (1160.34±273.75), MMP-9of theblood in bronchiolitis obliterans group(2872.15±737.04) Significantly increased(P<0.05), compared with control group(211.56±41.04), TIMP-1of thebronchoalveolar lavage fluids and blood in bronchiolitis obliteransgroup(144.55±24.84) was not show significant difference(P<0.005), compared withcontrol group（0.50±0.13；4.90±1.29）；MMP-9/TIMP-1in bronchiolitis obliteransgroup (14.71±2.53；11.14±2.78) was Significantly increased（P<0.01；P<0.05）。Conclusion Children with bronchiolitis obliterans, MMP-9and, The MMP-9/TIMP-1ratio in the bronchoalveolar lavage fluids and blood was significant increased andsuggest that extracellular matrix metalloproteinase-9(MMP-9) may be involved in the pathogenesis of the disease,imbalance of The MMP-9/TIMP-1ratio may becritical factor in the Fibrosis and remodeling of post-infectious obliterans bronchiolitisin children Objective To investigate bronchoscopic biopsy histological features inChildhood bronchiolitis obliterans and combined with clinical and imagingfindings,In order to enhance awareness of the disease, diagnosis and differential diagnosisMethod A retrospective analysis of13patients were clinically diagnosed BO，ranging from9to38months in age，10males and3females， the average of course ofdisease was8months， The main clinical symptoms of the patient in all werepersistent wheezing, shortness of breath, coughing and symptoms were sustained formore than six weeks. All the children underwent bronchoscopic biopsy (TBLB) andpulmonary high-resolution CT (HRCT) examination. the cellular profiles ofbronchoalveolar lavage fluids was measured by Induced sputum examination.in ninepatients, pulmonary function testing was measured in one patient.Results All patients had typical clinical Characteristics (TBLB) findings as follows:Peribronchiolar inflammation inflammation in13case (100%); bronchioles fibrosis in11cases (85%); epithelial cell necrosis in7patients (53%)；adjacent alveolar atrophy6cases (18%); HRCT findings as follows: Mosaic perfusion syndrome in allcase(100%)；bronchial wall thickening in3cases (23%)；scattered spots or patchyopacities in9cases (76%)；gas incomplete or consolidation atelectasis3cases (23%)；bronchiectasis in2cases (15%)； localized calcification in one cases (7%). Lavagecytology results of the analysis: increase of neutrophils in9cases (>38.0%), of whichseven cases were significantly higher (≥60%).13routine expenses fiber endoscopyShow endobronchial meningitis. A case had pulmonary function tests and the resultthat were consistent with obstructive ventilatory dysfunctionConclusion Peribronchiolar inflammation inflammation and bronchioles fibrosis on (TBLB) histological show is relatively characteristic to pediatric BO，combined withclinical and imaging findings， may be enhance the diagnosis and differentialdiagnosis.