Rapid Screening Of Gynecological Malignant Genital Tumorsin Extractive Electrospray Ionization Mass Spectrometry

Objective:1. Using Extractive Electrospray Ionization Mass Spectrometry (EESI-MS) inpatients with gynecologic malignancies vaginal secretions to obtain a validfingerprint;2. By obtaining a fingerprint to distinguish patients with gynecologicmalignancies and healthy control group;3. Find the difference between the mass peak gynecological cancer patients andhealthy controls, creating a fast, efficient and high-throughput detection methods forthe rapidscreening of Biomedical early cancer.Methods:Without Vaginal secretions complex pretreatment, using self-developedlaboratory extraction electrospray ionization mass spectrometry sample of vaginalsecretions in patients with benign diseases and gynecological cancer patients withrapid mass spectrometry analysis, set LTQ-MS detection mode positive ion mode, therange of the scanning detector is m/z50~2000, the ionization voltage was3.5kV,the ion transfer tube temperature of150℃, atomizing nitrogen pressure of1.0MPa,a lens voltage of80.0V, capillary voltage of7V. Using principal componentanalysis of these two groups of vaginal secretions fingerprint spectra data todistinguish.Results:1.Using EESI-MS technique, under conditions without sample pretreatment,successful vaginal secretions quick access to different fingerprint spectra.2.Cervical cancer patients and patients with benign gynecologic vaginalsecretion sample fingerprint in the relative abundance mass spectrum of the peak m/z66,81,102,143,332,248,497,817etc. are more prominent differences, especiallydifferences in the relative abundance of m/z143mass spectrum peaks is mostsignificant. Using principal component analysis can better to distinguish the twogroups. 3. Using PCA can successfully distinguish between patients and patients withbenign gynecologic disease endometrial cancer fingerprint spectra of samples ofvaginal secretions, and found m/z132,143,173,203,337,599,601, etc. the relativeabundance of mass peaks at a more prominent differences, especially differences inthe relative abundance of m/z143mass peak of the most obvious.Conclusions:1.Using EESI-MS technique, without the need for complex sample pretreatmentconditions, access to fingerprint spectra of different vaginal secretions.2.EESI complex matrices can be successfully ionized sample of vaginalsecretions, and can successfully obtain reliable fingerprint spectra data.3.Combined with the successful implementation of the PCA method ofendometrial cancer patients and patients with benign gynecological diseases cervicalvaginal secretions effectively distinguish quickly.