Effect Of Esmolol On SOD And MDA During Spinal Cord Ischemia/Reperfusion Injury In Rats

Objective：To observe the effect of esmolol on SOD and MDA during spinal cordischemia/reperfusion injury in rats, explore its neuroprotective effects on spinal cordischemia/reperfusion injury.Methods:72healthy male Sprague-Dawley rats, weighing200-230g, were randomlydivided into three groups. Sham operation group (group S n=8), ischemia/reperfusiongroup (group I n=32), esmolol group (group E n=32). group S exposed anddissociation infrarenal aorta after anesthesia. Group E intravenous infusion1mg/kgesmolol pre-anesthesia, intraoperative given intravenously200μg.kg-1.min-1esmololto the end of blocker. group I was intravenously normal volume saline. Ischemia30min, restore blood flow. I and E group divided into four subgroups. after thereperfusion6h,24h,3d,7d. four time periods(respectively designated as group I1、I2、I3、I4and E1, E2, E3, E4n=8each) with improved Tarlov score lower extremitymotor function. after score immediately decapitated while extracting arterial bloodtesting the activity of superoxide dismutase and the content of malondialdehyde;microscopy observe spinal cord histopathology.Result:(1) E1, E2group Tarlov score higher than I1, I2group, lower limb movementwas better than good in the ischemia group (P <0.05). However, E3, E4than thegroup I3, I4group score no significant difference (P>0.05).(2) E1, E2group electron microscope cases of spinal cord neurons wassignificantly better than I1, I2groups. The fewer of Karyopyknosis and necrosis.However, E3, E4group than I3, I4group in the electron microscope, no significantdifference in cell morphology.(3) E1, E2group the activity of superoxide dismutase and malondialdehydecontent was significant higher than I1, I2groups. E3, E4group than I3, I4, althoughhave little difference between groups, but not statistically significant (P>0.05) Conclusion:Esmolol can effectively improve SOD activity and lower MDA content in theacute phase of spinal cord ischemia/reperfusion injury, but for the delayed phaseeffect don’t effection...