Effect Of Extract Of Gynura Procumbens(Lour.)MERR On Malignant Phenotype Of U2-OS Cells In Vitro

Objective:To investigate the effect of Gynura procumbens(Lour.)MERR. Extract (GPE)on proliferation, apoptosis,migration and invasion of U2-OS cells in vitro.Methods:Different concentrations of GPE (5,10,20,40,80,160μg mL-1) were used onhuman osteosarcoma cell U2-OS for various time (24,48, and72h) in vitro. Theeffect of GPE on the proliferation of the U2-OS cells was investigated using MTTassays. Flow cytometry asssys was used to analysis the effect of GPE on theapoptosis of the U2-OS cells. Wound healing and Transwell invasion assays wereperformed to evaluate the migration and invasion abilities of U2-OS cells.Results:①After treated with different concentrations of GPE (5,10,20,40,80,160μg mL-1) on U2-OS cells for24,48, and72h, the proliferation of the cells were inhibitfor many degrees, and with the increasing concentrations and the treated time, theinhibition rate becomed higher than before.②After treated with different concentrations of GPE (10,20,40,80μg mL-1) onU2-OS cells for24h, the inhibition rate5.5%,7.6%,24.7%and37.94%weresignificantly higher than the inhibition rate0.18%in control group(p＜0.05).③After treated with40μg mL-1GPE on U2-OS cells for24h, the averagemigration rate (33±3)%and the average number of transmembrane cells (18±2) pervisual field were significantly lower than that (66±2)%and (46±2) per visual field incontrol group(p＜0.05). Conclusion:GPE could inhibit proliferation, migration,invasion and induced apoptosis inU2-OS cells in vitro, which occurred in a time-dose dependent manner，and it wasexpected to become a new drug to resist osteosarcoma.