Study On Determination And Pharmacological Activities In The Leaves Of Malus Baccata (Linn.) Borkh

Our study determined the content of phloridzin in the fresh leaves, unbarked fruits and barkof Malus baccata. The analysis was carried out on a Hypersil ODS2(250mm×4.6mm,5μm)column eluted with a mobile phase of methyl cyanides-water (25:75); The flow rate was1.0mL·min-1and column temperature was30℃. The detection wavelength was set at285nm.The results shows the content of phloridzin in the fresh leaves,unbarked fruits and bark ofMalus baccata were1.199%,0.117%and2.124%, respectively. The calibration curve ofphloridzin was linear in the range of20.0-100.0μg·ml-1with the average recovery of98.7%,100.3%,97.3%（n=5）and RSD of2.37%,3.27%,2.67%, respectively. The method is simple andaccurate and can be used for the quality control of Malus baccata..We measured the content of other flavonoids in of he fresh leaves Malus baccata. Theanalysis was carried out on a Hypersil ODS2(250mm×4.6mm,5μm)column eluted with amobile phase of methanol-0.2%phosphoric acid in water (methanol:0-40min,10%-50%;(methanol:40-50min,50%). The flow rate was1.0mL·min-1and column temperature was35℃.The detection wavelength was set at285nm. The injection volume was20μL. The content ofrutin, phlorizin, quercetin and phloretin was9.72mg·g-1,51.04mg·g-1,9.44mg·g-1and2.73mg·g-1, respectively. The method is simple and accurate and can be used for the quality controlof Malus baccata.Our Objective is to investigate the antioxidant effects of ethanol exyract of Malus baccataleaves (MBE) in vitro and study the protective effect on carbon tetrachloride-induced acute liverinjury in mice. The antioxidant effects of MBE were mearsureed by methods of DPPH, ABTS,FTC and TBA in vitro. The acute hepatic injury in mice induced by carbon tetrachloride.60ICRmice were randomly divided into control group, model group, Biphenyldicarboxylate group,and high, middle and low dose of MBE group, and there were10mice in each group.controlgroupserved as the control and was given0.5%sodium carboxymethylcellulose (CMC-Na) for14days. Group II served as tmodel group and was given1mL·kg-1body weight of CCl4(10%CCl4in olive oil, i.p.) at the last administration. Biphenyldicarboxylate was treated with thestandard drug biphenyldicarboxylate（150mg·kg-1body weight, p.o.）for a period of2weeks.Groups low, middle high were administered an oral dose of100,300, and500mg·kg-1bodyweight of ethanolic extract for2weeks, respectively. Additionally,1h after administration ofeach groups, alll groups were administered1ml/kg body weight of CCl4(10%CCl4in olive oil,i.p.) at the last treatment on the day14, except control group. After6h, Animals were sacrificed by bleeding and blood was collected to measure alanine aminotransferase (ALT) and aspartateaminotransferase (AST). The clear supernatant from liver samples was used for determination ofsuperoxide dismutase (SOD), malondialdehyde (MDA), and glutathione (GSH). The resultsshowed that MBE possessed strong antioxidant activity in vitro. MBE significantly decreasedthe levels of ALT and AST, levels of GSH and SOD were improved and the level of MDA wasprevented. The hepatoprotective activity of MBE may be due to its free radical-scavenging andantioxidant activity, resulting from the presence of some flavonoids and phenolic compounds inthe extracts.To study hypolipidemic and hypoglycemic effects of Malus baccata total flavonoids onSTZ-induced diabetic mice. Diabetes mellitus was induced in mice by intraperitioneal injectionof streptozotocin (STZ,100mg·kg-1). All the mice were divided into five groups randomly,including normal control group, diabetic model group, metformin group (250mg·kg-1), MBEhigh dose (500mg·kg-1) and low dose group (300mg·kg-1). All the mice were intragasticadministration for4weeks, weight, food and fluid intake were monitored every day, fastingblood glucose (FBG) was measured3times during4weeks. We also tested the effect of MBE toOGTT. At the end of the experiment, liver, kidney, spleen and thymus samples were moved andweighed to calculate the tissue index. All the blood was collected to evaluate blood lipid. T.heMBE high dose could significantly reduce the FBG and the area of AUC, low dose couldimprove the FBG in some extent. Compared with model group, the high dose and low dose hada significant decrease in the level of TG and TC, ameliorating the level of HDL-c. Our studyfound that the injured organs could be repaired. MBE can expressly improved glucose and lipidsabnormality.