Estrogen Related Receptor Gamma Expression In Breast Hypertrophy And Significance

Every woman wants to have a pair of breast of rich and full of elasticity. Which makewomen to be smooth, mellow and graceful curve. It seems to have passed all of thesebreast by hypertrophy patients. Huge breast shape and deformed that they suffer physicaland psychological pressure. breast weight is too heavy to cause neck, back and shoulderpains, The arthritis and cervical spine even severe cases can cause hunchback and thoracicdeformity. Huge breast also caused double arm numbness and paraesthesia local nervecompression. Degree of breast prolapse also often leads to excessive breast below prone torash and skin ulcers, Even durable Etc. Breast hypertrophy patients they are not willing toparticipate in social activities, buy the properties clothes, unable to take part in physicalexercise and so on. It’s seriously affecting the patient’s quality of life and mental health.Since the early twentieth century, patients attempt to optimize aesthetic and surgicaloutcomes in reduction mammaplasty have prompted constant development and revision ofsurgical techniques. Breast reduction surgery is also the fastest way of solving patient’sbreast hypertrophy and deformity. but surgery can lead to patients with trauma andpostoperative scar and concern. The expensive cost also make some patients hope and retrogression. There for, find the cause of breast hypertrophy is very necessary. But thebreast hypertrophy specific pathogenesis is still unclear. There are two opinions for breasthypertrophy as to cause. One is that may be associated with increased breast tissue topicalestrogen; the other one may be associated with target cells increased susceptibility toestrogen. This experiment from the aspects of peripheral blood levels of the hormone andestrogen receptor of breast hypertrophy.The patients from this study the selection of clinical patients with mammaryhypertrophy and suspension of normal breast volume in patients with breast. the clinicaldata of two groups of patients with detailed records, carefully calculated body mass index,physiological cycles for each patient and breast volume. ruled out due to obesity as a resultof adipose breast hypertrophy and patients with other systemic diseases. According to thephysiological cycles patients are dilided into follicular phase, ovulation and luteal phase.Collect each consistent with the experimental study in patients with peripheral blood,17beta in peripheral blood plasma estradiol indexes immunoradiometric assay, measurementsusing SPSS16.0statistical software version analysis processing, body mass index betweenthe two groups compared by t test, peripheral blood hormone level index compares theMann-Whtney U statistical analysis, P <0.05for the result difference is statisticallysignificant.The experimental results for body mass index in hypertrophic breast group (n=95)patients and normal breast volume (n=84) with no statistically significant difference (P=0.0.9462>0.05), prove that our choice of the experimental group and normal controlgroup patients sources can be ruled out due to the changes of obesity causes of breastvolume, behind to further provide the basis of experiment.17beta estradio in hypertrophicbreast group (37cases) and normal breast volume(27cases) in Follicular phase forstatistical analysis,17beta estradiol in peripheral blood between the two groups there wasno statistically significant difference (P=0.3029>0.05).17beta estradio in hypertrophicbreast group (32cases) and normal breast volume(31cases) in oviposit period forstatistical analysis,17beta estradiol in peripheral blood between the two groups there wasno statistically significant difference (P=0.8160>0.05).17beta estradio in hypertrophic breast group (26cases) and normal breast volume(26cases) in Luteal phase for statisticalanalysis,17beta estradiol in peripheral blood between the two groups there was nostatistically significant difference(P=0.5526>0.05). Physiological cycles inpostmenopausal patients with clinical cases are rare, cannot undertake statistical analysis,This experiment study can not some of the patients in this study. Microscopic observationon HE stained sections showed that the remaining lobule structure within the lobulemesenchymal tissue hyperplasia obviously, the interlobular collagen fiber hyperplasia ofobvious swelling and acinar epithelial hyperplasia of also have. The lobular fiberconnective tissue stroma by excessive proliferation and the lymphocytes can be seen in theirregular distribution, the breast tissue catheter expansion and ductal epithelial hyperplasiaof the nipple is obvious. Normal breast tissue within the breast lobules and interlobularloose fibers without hyperplasia, clear boundaries and no epithelial hyperplasia. In breastinvasive ductal carcinoma as a positive control, anti-ERRγ antibody Immunohistochemicalstained: the mammary hypertrophy breast tissue in comparison with normal breast tissuevolume that the lobular, catheters and acinar epithelial ERRγ strongly expressed, brownishyellow nucleus, cytoplasmic medium, the cells arranged in neat and orderly, honeycombcell size and shape is more consistent, round or oval nucleus, chromatin distribution. Thenormal breast tissue volume of mammary gland flocculus, catheters, acinar epithelialERRγ positive cells expressing the weaker. Negative control groups (no anti ERRγIgG)no ERRγ expression. Using Image Pro plus (IPP)6.0image analysis software for chemicalquantitative immunohistochemical analysis, the final results of10sets of data are shownin Table I、Table II、and Figure IV, Comparison between the two groups by the singlefactor analysis of variance (one-way ANOVA), P <0.05, Compared the breasthypertrophy group with normal breast volume group were statistically significantdifference in every index. Stewards to GAPDH gene as the internal standard, Breasthypertrophy group ERRγ express a stripe of mammary gland expression than normalbreast volume group was obviously enhanced in the PCR reaction. breast hypertrophygroup within the breast tissue ERRγ expression quantity compared with normal breastvolume group P>0.05, differences has obvious statistical significance in the RT-PCR reaction.The results demonstrated that after the exclusion of body weight, obesity andsystemic effects of other diseases, physiological period in any period. Breast hypertrophygroup and normal breast tissue volume group compared with patients with peripheralblood17β-estradiol index showed no significant difference, and each of the femalehormone levels were lower than the standard clinical diagnosis of breast cancer, Indicatingno clear etiology of breast hypertrophy relationship with estrogen levels in peripheralblood. HE stained sections showed that breast hypertrophy group breast tissue than normalbreast volume compared to the mammary gland, catheter and interstitial hyperplasiaobviously. Immunohistochemical stained showed that breast hypertrophy and normalbreast volume data set of each group was significantly lower than the group of breastcancer, breast hypertrophy group compared with normal breast volume group, breasthypertrophy group of positive cells expression rate (83.52%) was obviously higher thannormal breast volume group (25.94%); In PCR and RT-PCR breast tissue in the amount ofERRγ expression in breast hypertrophy group is significantly higher than normal breastvolume, RT-PCR result shows ERRγ in breast hypertrophy group within the breast tissueand normal breast volume difference has significant statistical significance (P>0.05).above results shows that breast tissue in breast hypertrophy group ERRγ expressionincreased, lead to mammary gland hyperplasia and breast size.