The Study Of Combination Of Macs With DGC On Sperm Optimization In Male Infertility

Objective:The combination of density gradient centrifugation (DGC) and magnetic activated cell sorting(MACS) is a emerging method of sperm selection. In this study, we optimized spermatozoa by using the combined technique. Routine semen analysis and further detection of sperm DNA integrity identified by sperm chromatin dipersion text (SCD) were applied to evaluate the combined technique in optimization of semen quality in male infertility.Method:Semen samples from20infertile male outpatients of Andriatrics department in The First Affiliated Hospital of Guangxi Medical University were analyzed. Another semen samples from20health donors were analyzed for comparison. The semen samples were objected to separate on DGC followed by MACS. Sperm density, viability, motility, and motility parameters of sperm before and after selection were detected by computer-assisted semen analysis (CASA) system. The changes of sperm morphology were observed by Papanicolaou stain, and the change of DNA integrity of sperm before and after were identified by SCD test.Results:①Before selection, the semen samples of male infertile patients displayed poor quality in term of lower viability, a-class motility, normal morphology rate and higher DNA injury rate compared with that of health donors(P<0.01).②Sperm density, viability, a-class motility and the number of sperm with normal morphology was significantly increased in two groups after selection (P＜0.01).③fter selection, motility parameters such as VCL VAP、BCF were significantly increased in two groups (P＜0.01),while LIN、STR、ALH、WOB decreased (P＜0.01). VSL of health honors group increased (P＜0.05),while VSL of male infertility had no significance when compared with raw semen(P>0.05).④DNA injury rate in two groups decreased significantly after sperm selection (P＜0.01)Conclusion:As a new method of sperm selection, the combination of DGC and MACS could optimize superior spermatozoa in terms of sperm density, viability, motility, morphology and DNA structure.