| Objective To investigate the impact of abdominal hypo-fractionation irradiation on liver damage in rats so as to provide a reference for its clinical application.Methods A total of100Wistar rats were equally randomized to five groups as control,2Gy,4Gy,6Gy and12Gy group, the corresponding fractionated doses were offered. Liver functions were examined at the2nd,4th,6th,8th and10th week after radiation. Morphological changes were observed by HE staining. Expressions of Bcl-2and Bax were examined by immunohistochemical technique.Results In all irradiation groups, hepatocellular swell, degeneration, necrosis and even hepatic fibrosis could be seen. The differences of G P T, G O T were significant among the groups and different time points (F=11.833-781.972, F=20.857-264.692, P<0.001). Interaction effects showed significant differences between fractionation doses and observing time points (F=2.139-27.550, P<0.001). Expressions of Bcl-2and Bax were significantly different between each group (F=211.607,116.577; P<0.001), and between each time point (F=5.032,4.385; P<0.001).Conclusion Abdominal hypo-fractionation irradiation may cause radiation damage to rat liver, being dose-and-time dependent. Up-regulation of activating apoptosis protein Bax and down-regulation of inhibiting apoptosis protein Bcl-2may involve in the process. |
PDF Full Text Request