Expression Of Toll-like Receptor(TLR4), TLR9and DC-specific Intracellular Adhesion Molecule-3rgabbing Non-integrin(DC-SIGN) In Condyloma Acumlnam Lesions And Its Clinic Pathological Significance

Objective To investigate the expression and clinic pathological significance of TLR4,TLR9and DC-SIGN in primary and recurrent condyloma acuminatum(CA) lesions．Methods An immunohistochemical method using streptavidin-peroxidase (SP)was performed to detect the expressions and distribution of TLR4, TLR9andDC-SIGN in tissue specimens obtained from the recurrent CA lesions of30patients,Primary CA lesions of30patients, And from the foreskin of20normal humancontrols. Consult Fromowitz standard for evaluation to assess each specimen,analyzing the result of TLR4, TLR9and DC-SIGN in Primary CA lesions andrecurrent CA lesions with Student’s t test and spearman rank correlation in spss17.0statistical software.Results the positive expression rate of TLR4, TLR9and DC-SIGN in primaryand recurrent CA lesions were all100%, but the data in normal control tissue is5%,10%and5%. The expression levels of TLR4, TLR9and DC-SIGN in primary andrecurrent CA lesions were significantly higher than those in normal control tissue(all P<0.001), and the cells expressing TLR4, TLR9or DC-SIGN were mainly locatedin the basal and spinous layer in CA lesions. There was no significant differencebetween the expression of TLR4, TLR9or DC-SIGN between primary and recurrent CA lesions (all P>0.05). A positive correlation was found between the expressionlevels of TLR4, TLR9and DC-SIGN in primary and recurrent CA lesions (rs1=0.489，P<0.05;rs2=0.475，P<0.05).Conclusion The overexpression of TLR4, TLR9and DC-SIGN in primary andrecurrent CA lesions probably play an important roles in the occurrence andrecurrence of condyloma acuminatum.