The Effect On Silence Of Gene By JARID1B RNA Interference In MCL

Objective: To study the state of JARID1B, LSD1, H3K9, H3K4, in mantle cell lymphoma(MCL) and study alteration of cell proliferation, apoptosis and histone methylation and acetylationafter silence of JARD1B gene by small interfering RNA(siRNA) in Jeko-1cells.Methods：1.The expression of JARID1B, LSD1, histone H3K9and H3K4methylation in MCL detected by Immunohistochemical.2. A siRNA segment targetingJARID1B gene was designed and transfected into Jeko-1cells byLipofectamineTM2000. JARID1B mRNA transcription level was detected by RT-PCR3. Cell growth curve was draw by MTT. Cell apoptosis was measured by Flowcytometry.4. The expression of Bcl-2、procaspase-3、C-myc and JARID1B proteinand the expression of histone methylation of H3K4and histone acetylation of H3、H4were detected by Western Blot.Results:1. The expression of JARID1B, LSD1was increased and histone methylatedH3K4were decreased in MCL compared to proliferative Lymphadenitis （p＜0.05）.2. JARID1B mRNA and its protein were inhibited after transcription of the siRNA ofthis gene.3. JARID1B siRNA inhibited cell growth and cell apoptosis in dose andtime-dependent manner in Jeko-1cells line. IC50was60nM.4. The expression ofBcl-2, procaspase-3, C-myc was decreased after transfection of the gene for24hours.5. JARID1BmRNA upregulated histone methylated H3K4and histone acetylation ofH3. The change of histone acetylation of H4was not seen.Conclusions:1.JARID1B and LSD1might be one of the Pathogenesis of MCL sincethey are higher expression which results in low expression of H3K4.2.JARID1BsiRNA inhibites cell growth and induces cell apoptosis in Jeko-1cell line.3. JARID1B siRNA upregulates histone methylated H3k4and acetylation ofH3. It might be a new therapeutic target in MCL.