Properties Study Of Silk Fibroin-doped Electrospun Polyurethane Nanofibers

Polyurethane (PU) is widely used in biomedical filed as the biomaterial. As it has goodbiocompatibility and excellent mechanical properties, but its biocompatibilty need to beenhanced. Because the formation of thrombus and the earlier inflammatory has limited itsapplication in clinic. And many research has depended on how to improve it’s biologicalcompatibilitySilk as a natural protein is very extensive in china. Silk contains 70% of silk fibroin,which is a natural protein, it contains many amino acids which is necessary for the body. Wecan blend polyurethane and silk fibroin to make electrospun nanofiber, which can improve thebiocompatibility for the blend menbranes, as the silk has excelent biocompatibility. Heparin isan anti-clotting normally present in animal liver which has strongly acid. When SFPs wereadded into the PU polymer solution to fabricate the SFP/PU composite nanofibrous mats,which can increase the anti-clotting ability of the blend membranes.In this study, we systematically investigated the processing parameters on themorphology of the electrospun ultrafine polyurethane nanofibers. we show concentration of15wt% favored the formation of continuous polyurethane nanofibers with the applied voltageof 20 kV, flow rate of 1ml/h and the collection distance of 20 cm. Whereas the mixed THFand DMF solvent with a volume ratio of 5:5 was beneficial for the evaporation of solvent andthe fabrication of smooth and uniform PU nanofibers.In this study, we systematically investigated the heparin (HEP) which was blend with PUby the drug-carrier of SFP electrospun polyurethane nanofibers. we firstly investigated theeffect of processing parameters including concentration and the volume ratio of DMF andTHF（5:5）in the mixed solvent on the morphologies of PU nanofibers. Then, under theoptimized electrospinning parameters, SFPs were added into the PU polymer solution tofabricate the SFP/PU composite nanofibrous mats with different SFP loading. Then wesystematically investigated the release of HEP, and the growth speed of rat fibroblast indifferent nano blend membranes.