Anti-oxidative Activity Of Erodium Stephanianum Willd. And Terminalia Chebula Retz.

Objective To study the anti-oxidative activity of Erodium stephanianum Willd. and Terminalia chebula Retz. in vitro and in vivo and determine their total phenolic content, flavonoid content and flavonol content.Method Anti-oxidative activity of E. stephanianum and T. chebula in vitro was evaluated by DPPH free radical scavenging method. The total phenolic content, flavonoid content and flavonol content were determined by Folin-Ciocalteu colorimetric method.60 male mice were randomly divided into normal control group, model control group, high dosage group, middle dosage group, low dosage group, and positive control group (silybin). The mice in the above groups were fed for 5 days. The high dosage group, middle dosage group and low dosage group were pretreated intragastrically with 2.0,1.0,0.5 g/kg water extract of E. stephanianum. The Positive control group was pretreated intragastrically with 0.1 g/kg silybin. The normal control group and model control group were given the same volume of physiological saline. On the 5th day of feeding, the mice except normal control group were given intraperitoneally 0.15 mL/lOg 0.1% carbon tetrachloride. The normal control group was given the same volume of physiological saline. Twenty hours after the intoxication, the serum AST and ALT activities, SOD, GSH-Px activities, and MDA content in the liver were measured in each group mentioned above.60 mice were randomly divided into normal control group, model control group, high dosage group, middle dosage group, low dosage group, and positive control group (cimetidine). The mice in the above groups were fed for 5 days. The high dosage group, middle dosage group and low dosage group were pretreated intragastrically with 1.0,0.5,0.1 g/kg water extract of T. chebula. The Positive control group was pretreated intragastrically with 0.05 g/kg cimetidine. The normal control group and model control group were given the same volume of physiological saline. On the 5th day of feeding, the mice except normal control group were given intraperitoneally 0.1 mL/lOg absolute ethyl alcohol. The normal control group was given the same volume of physiological saline. One hours after the intoxication, the gastric ulcer area and the serum SOD activities were measured in each group mentioned above.Result The water extract of E. stephanianum showed strong DPPH free radical scavenging activity in vitro. The high dosage group, middle dosage group, low dosage group significantly decreased carbon tetrachloride induced acute hepatic injury with increasing the activities of ALT and AST in serum and reduced the indicators of oxidative stress in the liver such as MDA, increased the activities of GSH-Px and SOD. The water extract of T. chebula showed strong DPPH free radical scavenging activity in vitro. The high dosage group, middle dosage group, low dosage group significantly decreased absolute ethyl alcohol induced the gastric ulcer area and increased the activities of SOD.Conclusion:Based on the results described above, we speculate that the water extract of E. stephanianum has the potential to play a role in the hepatic prevention of oxidative damage. In addition, the water extract of T. chebula has the potential to play a role in the gastric ulcer prevention.