The Effect Of PHF20, CYHR1, LOC514211, MAN1A1, Spatc1 Gene Mutation On Milk And Reproduction Traits In Dairy Cows

The objective of this study were to identify and confirm the SNP associated with milk production and reproduction, whose SNP were selected from Chinese and British dairy cows based on 60k SNP-Chip. The research was conducted in laboratory of animal genetics, breeding and reproduction in Huazhong Agricultural University, Wuhan City, Hubei Province, People’s Republic of China, starts on March 2010-December 2012.470 head Dairy Cows and 35 head water buffaloes have been used in this research, DNA was extracted from the blood samples, PCR-RFLP has been applied to genotype these samples. Analysis of Variance (ANOVA) has done using the General Linear Model (GLM) procedure from SAS 9.13. A value of P< 0.05 was regarded as significant. The statistical model used was Yijk=μ+hj+Pj+Qk+NI+eiijkI. Used in analyzed the association of SNP of genes with milk yield (305 DM, Total Milk Yield), milk quality (fat percentage, protein percentage, Total Milk Yield fat, Total Milk Yield protein) and reproduction traits (DFS, AOC, BW, DO and CI).Five SNPs were identified from five candidate genes. SNPs from five candidate gene in cows were polymorphic in dairy cow, whereas in Buffalo were non-polymorphic.4 of SNPs located on PHF20, CYHR1, LOC514211 and SPATC1 were in Hardy-Weinberg disequilibrium, while one SNP located on MAN1 A1 was in Hardy-Weinberg equilibrium.Five SNPs from five candidate gene were identified. SNPs genotypes of five candidate gene PHF20, CYHR1, LOC514211, MAN1A1 and SPATC1 had significant difference in milk reproduction and reproduction traits on Dairy Cow. The mutations of five candidate genes results in significant changes of milk production and reproduction traits in dairy cows. The results indicated that TT genotype of PHF20, A A and GG genotype of CYHR1, TC and TT genotype of LOC514211, AC genotype of MAN1A1 and also AG genotype of SPATC1 candidate gene ware potential marker on both milk production and reproduction traits. There are similar results found in previous SNP-Chip with present study.305 DM AA genotype of MAN1A1 was dominant genotype in both studies.Further research must be conducted. Validation of SNP identified in the present study must be confirmed in other larger populations. A greater number of SNP and a greater number of candidate genes must be evaluated in order to properly identify significant marker association and identify SNP that account for the largest degree of variability for the trait of interest.