The Study Of Rice Plasma Membrane Proteome During The Infection Of Magnaporthe Oryzae

Rice blast, caused by the causal agent Magnaporthe oryzae (M. oryzae), and emerges as a major threat to cereal production word-wide, which causes about 15% yield losses annually. M. oryzae is a hemibiotrophic pathogen. Similar to other hemibiotrophic pathogens, M. oryzae inhabits the extracellular niche at early infection stage and switches to necrotrophic phase following the collapse of host cells. Interestingly, during the interaction of rice and M. oryzae, plant Plasma membrane (PM) encloses fugal invasive hyphae, forming the plant-derived extrainvasive-hyphal membrane (EIHM). In addition, it has been shown that plant PM-derived biotrophic interfacial complex (BIC) that lies outside of the PM and cell wall of the fungus, preferentially accumulates pathogen-delivered effectors. In either case, plant-derived PM acts at the front line than any subcellular organelles in coping with pathogen invasion. Here, we utilized quantitative mass spectrometry to explore the rice PM protein composition and dynamic changes during M. oryzae infection, in order to unravel the roles of plant PM in plant and microbe interaction.We report thus far the largest rice PM Proteome dataset with 3,906 identified proteins, among which 484 proteins were differentially expressed after M. oryzae infection. One third of the identified proteins are predicted to have at least one transmembrane domain. Half of the identified proteins are predicted to have binding functions and over one third of the proteins have enzyme-related functions. In addition, GO analyses revealed that abscisic acid (ABA) and cytokinin (CKs) signaling were sequentially activated after M. oryzae infection in rice. We found that the activation of ABA signaling and the suppression of rice immune response occurred at early infection stage, while the activation of CKs signaling, the upregulation of sugar transporter genes expression and the nutrient efflux of infected rice cells occurred at later infection stage.Thus, we further propose that M. oryzae activates ABA signaling to repress rice immune signaling for initial invasion and redirects nutrient efflux of infected cells for massive growth at later infection stage.