The Expression Of Taurine Synthesized Key Enzymes And Taurine Transporter In Tissues Of Ovis Aries

Taurine (2-aminoethane-sulfonic acid) was first identified and isolated from the bile of the ox, from which it derives its name. Taurine is one of the most abundant amino acids in many organs, acts as a cell cytoprotectant, and plays an important role in the modulation of homeostasis, intracellular free calcium concentration, hormone secretion and development of sexual glands. Taurine deficiency will result in urinary system diseases, endocrine system diseases and cardiovascular system diseases. It has been demonstrated that taurine had beneficial effects in diabetes, hypothalamus-pituitary system pathological change, stress-induced neuronal damage, liver disease and other diseases.Although taurine has many physiological function, the information of taurine biosynthesis and its action in ovis aries are still unclear.In the present study, by the methods of RT-PCR, RT-qPCR,Westen-blot and ELISA, the expression of CDO, CSD and TAUT in ovis aries hypothalamus, pituitary, liver, thyroid, adrenal gland, ovarian and testicular, and taurine concentration in those tissues were tested. The results will provide basis for laboratory’s further study on the effect of taurine in ovis aries and application of taurine in ovis aries production.The results showed that CDO, CSD and TAUT mRNA and protein were expressed in hypothalamus, pituitary, liver, thyroid, adrenal glands, ovarian and testicular tissues of ovis areis. The expression levels of CDO mRNA were decreased from hypothalamus, liver, pituitary, thyroid, adrenal gland, testis to ovary, CSD mRNA expression levels were decreased from adrenal gland, liver, ovary, thyroid, hypothalamus, pituitary to testis, and TAUT mRNA expression levels were decreased from ovary, liver, hypothalamus, thyroid, testis, pituitary to adrenal gland. The results also showed that taurine exists in ovis aries hypothalamus, pituitary, liver, thyroid, adrenal gland, ovary, testis and serum. The levels of taurine in ovis aries tissues were decreased from testis, ovary, liver, hypothalamus, adrenal gland, thyroid, pituitary to serum.The results indicated that taurine can be biosynthesized through CDO-CSD pathway in ovis aries liver, hypothalamus, pituitary, thyroid, adrenal, testicle, and ovary. However, the level of biosynthesized taurine may not meet the need of organ function, and may obtain partly from the blood by taurine transporter. The present study also indicated that taurine may play an important role in ovis aries liver and endocrine glands, but the exact function need to be further investigated.