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Study On Soiless Culture And Rapid Propagation Of Alpinia Pumila

Posted on:2017-03-27Degree:MasterType:Thesis
Country:ChinaCandidate:Y Y HuangFull Text:PDF
GTID:2283330485972319Subject:Horticulture
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Alpinia pumila belongs to the family of the Alpinia L. of Zingiberaceae. Alpinia pumila is a wild low-herbage, which can be used as potted plant indoors and ground cover plant in urban landscape. Experiments were conducted for shade tolerance, soilless culture, tissue culture and rapid propagation of Alpinia pumila. It’s very significant to find the most appropriate light intensity, substrate, hydroponic nutrient solution formula and media component of Alpinia pumila for using it better, which can enrich the diversity of potted plant and ground cover. The results were as follows:1. Compared to full light(0%) group, the research investigated adaption of Alpinia pumila to different sunlight intensity including three shading levels in 50%, 75% and 90% by set up shade net artificially. The research showed that the suitable shading level for Alpinia pumila is between 75~90% of full light and ornamental value could be maximum in the degree of 90%(about 5000 lux on a sunny day); it’s not unfit for budding over 75% shading levels. However, the shading level should be set between 50~75%(about 10000~20000lux on a sunny day) in order to obtain the biggest breeding efficiency and the fastest growth in commercial production.2. According to the results from different proportion of mixed substrate, the optimal proportion of mixed substrate for Alpinia pumila was peat: green waste: fine sand: perlite: vermiculite=1:8:1:1:1. Alpinia pumila cultivated in this mixed substrate could maintain high ornamental value: more new leaves, deep green in color, obvious streak, glossy surface, less diseases and pests, strong underground roots and strong tiller capacity. As the main component of ideal substrate, green waste is not only abundant, cheap, light and clean, but also has a highest quality for the cultivation of Alpinia pumila, which is good for production and transportation of potted plant.3. The experiment, which was based on the evaluation of growth condition of Alpinia pumila with different treatments by four nutrient solutions and concentrations in 90 days, showed that 1/4 dose of Hoagland nutrient solution is the best nutrient solution. That solution was not make plants maintain the best state but fit for hydroponic production for saving the cost. However, compared with 1/4 dose of Hoagland nutrient solution, 1 dose of Dewoduo formula was much better for family hydroponics, because Dewoduo nutrient solution could be obtained directly in market and operated easily.4. Taking the stem apexes or the stems with bud of Alpinia pumila as explants, the optimal mediums of shoot induction, subculture multiplication and rooting were studied by adding the different plant growth regulators based on MS medium. The results showed that the ideal medium of shooting induction was MS+6-BA6.0mg/L(the same units as follows) + NAA0.1 with the rate of 52%. The proliferation coefficient of multiple shoots reached at 3.56-fold with MS+6-BA4.0+NAA0.1+TDZ1.0. And 1/2MS medium containing 0.5 mg/L NAA served as the optimal rooting medium.
Keywords/Search Tags:Alpinia pumila, wild plants, shading, substrate optimization, hydroponics nutrient solution, rapid propagation
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