Research On The Genetic Diversity And Liquid Fermentation Conditions Of Auricularia Auricular From Qinba Mountains

Auricularia auricular, a kind of fungus, is commonly taken as functional food in China. Auricularia auricular is abounding in Qinba Montains with plenty of wood resouces. In order to screen the good strains and keep the germplasm resources, the genetic diversity of Auricularia auricular was evaluated in Qinba Mountains. Furthermore, the liquid fermentation conditions of Auricularia auricular were also studied, which could be helpful for the liquid seed producing and its large scale cultivating.In this study, 45 strains of Auricularia auricular from Qinba Mountains were adopted. Genetic diversity among different strains was studied by analyzing the morphological characters, Esterase Isozyme, ITS sequence and incompatibility reaction. The liquid fermentation conditions of strain Er 261 were also studied by response surface methodology.1. The biological characteristicsThe biological characteristics of 45 Auricularia auricular strains were studied by the method of tissue culture. The results showed that biological differences were shown in the mycelium morphology, liquid morphology and agronomic characteristics. It can be concluded that the Auricularia auricular germplasm resources is rich and diverse in Qinba Mountains.2. The genetic diversityAuricularia auricular strains were verified by esterase isozyme, ITS sequence analysis and incompatibility reaction. The results showed that 45 Auricularia auricular strains can be classified into 4 categories: Strains Er 266, Guoxing 3#, Fenliu, A55, Er 5#, Fensi, Guoxing 2#, Ye 1, Er 1#, Guoxing1#, Erdongmei 1#, Gaochan 1#, Er 3#, Xiner 5#, Xinke 1#, Muer(D), Er 256, Xinke, Er 3-18, Xinke 6#, Xinke 9#, Erju 3#, Er 268, 112 dong, Er 91, Xinke 4#, LY16#, Shangluo Li, Er 801, Er 261, 36 Hao, Er 238, Er 629 and Er 913. Strains QD4#, QD3#, Xinke 5#, Au29, RF201, Er 97-2, Er 235, Er 269 and Shennong A8. Strain Er 2. Strain Gan 1. The rich genetic diversity could be elicited from Auricularia auricular strains.3. The liquid fermentation conditions of Er 261The suitable liquid fermentation conditions of strain Er 261 were optimumed by the single facter and Box-Benhnken response surface method. The best carbon source was glucose, and bean powder as the best nitrogen source. The best fermentation conditions were as follows: inoculation quantity 10%, pH 5.85, temperature 30?C, 100 mL of culture medium in 250 mL shake-bottle, rotate speed 160 r/min. The mycelial biomass of Auricularia auricular was 15.87 g/L with the relative error was 2.06%, which was better than the theoretical value 15.55 g/L. The response surface method is effective and feasible for the liquid culture conditions optimization of Auricularia auricular strain Er 261.