| 165 SPF ICR mice were assigned to three groups:the control group with 0.1 ml/10g norm 1 saline, and the CP groups with 0.6mg/kg and 6mg/kg respectively.After intraperitoneal injectio-n of cyclophosphamide for 7 days, the mice were fed for another 14 days, and the whole t-rial period was 21 days.4 mice in each group were randomly selected and sample-d at 1st day,4th day,7th day,14th day and 21st day. The mice were sacrificed and the spleens w-ere weighed to calculate the orgn index, and the slices of spleen slices were made to observe th-e morphological changes. Dynamic changes of lymphocytes subsets, cell cycle and apoptosis in s-pleen were determined by flow cytometry.Compared with control group, no significant changes can be found in the organ index of s-pleen in CP I and CP II group. Histologically, spleens in CP II group appeared the decrease of f-ollicular volume and lymphocytes number. The result determined by flow cytometry showed that, At 4th day and 14th day after the first administration, the percentage of CD 19+B lymphocytes was significantly lower than that of the control group in CP II group (P<0.05). When compared with the control group, the percentages of CD3+ã€CD3+CD4+ and CD3+CD8+T lymphocytes of spleen had no significant changes at 21st day in CP I and CP II group (P> 0.05). The percentage of apoptotic splenocytes was increased in CP II group than that in control group. The cell cycle was arrested in S phase (P<0.05) in CP I groups at 7th day. The cell cycle was arrested in G2+M phase (P<0.05) in CP II groups at the first day.This study suggested that 0.6mg/kg cyclophosphamide could not cause significantly affection in spleen, and 6mg/kg cyclophosphamide could induce obvious pathological damage in spleen, the decrease of the CD19+ percentage and the increase of apoptotic splenocytes and G2+M phase cell. After intraperitoneal injection of cyclophosphamide for 7 days, the above indicators in 0.6mg/kg CP group gradually got back to normal. |
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