Studies On The Key Technology Of Standardization Cultivation Of Leonuri Herba In Sichuan

Leonuri Herba (YMC) is the fresh or dried aerial part of Leonurus japonicus Houtt. (Labiatae). YMC have the effects of promoting blood flow for regulating menstruation, diuretic swelling, and detoxification. It is a herbal material commonly used in irregular menstruation, dysmenorrhea, amenorrhea, lochia, edema, oliguria, sore swollen poison.This project is aimed at the key technology standardization cultivation of YMC and germplasm resources from Sichuan Province. It is provided a fundamental basis for the building of GAP base for YMC.1 The key technology standardization cultivation of YMCYMC are distributed throughout the nation. It is grown in the wild or semi-wild; and have a wide range of germplasm, a mixed genetic population, and great differences in the quality and yild. Thus, we studied on the growth behavior and cultivation technology of YMC, and realized the goal of transferring the behavior from wild to cultibated in Sichuan Province.By performed in a variety of terrain and weather conditions in Sichuan Province such as Lezhi、Zhongjiang、Qionglai、Pengzhou、Mianning、Huili、 Puge, we have measuried its yild, active ingredient contents. According to the comprehensive situations of dirrerent cultivation bases, we builded the GAP base for YMC in Mianning County, Liangshan Prefecture, Sichuan Province.On this base, the optimal time of spring sowing for YMC is mid-B, and not more than C; the harvesting period is from late O to early P. Autumn sowing in late D to mid-E is best, no longer than the end of E, and harvest in S next year is most appropriate. Bunch planting cultivation is used. The distance between two caves is G2cm. The seeds each acre need are Ig. It is necessary to fertilize with compound fertilizer per acre (N:P:K=a:b:c) Lkg before seeding. It is time to weeding, fertilize when entering the jointing stage and the plants appearing square stems. After harvest, the best way to make the medicine dry is low-temperature drying process. However, according to the realities of situation, places of origin dried under the sun is more applicable. By studying the above key technologies cultivation of the GAP base, for the first time, we developed the cultivation SOP for YMC.We increased the microscopic identification of powder, heavy metals and harmful elements and organochlorine pesticides in our study under the quality standards of "Chinese Pharmacopoeia" 2010 edition. The item further enhanced the quality standards of YMC, and was beneficial to the safety and effect of YMC.2 YMC germplasm resourcesWe collected the seeds of YMC in a total of 32 copies from 19 provinces, containing YMC, Leonurus tataricus Linn. (XAYMC), and Leonurus sibiricus Linn. (XYYMC) from the wild and market. And stored these seeds in indoor temperature and low humidity (seed storage cabinet’s temperature,4℃ 10℃; humidity,30% to 40%). Meanwhile, builded the Germplasm nursery in the GAP base for YMC.By studying a variety of items such as grain weight, germination rate, agronomic traits, units of measurement area yield, assay of active ingredient contents, we found that (1) XYYMC have a low yields and low assay of Stachydrine hydrochloride and Leonurine hydrochloride, so, it’s unsuitable for XYYMC to use as YMC; (2) the concentration of Stachydrine hydrochloride and Leonurine hydrochloride for XAYMC is higher than XYYMC and closed to YMC; while high yield for XAYMC, considered researching XAYMC as a new material for YMC; (3) the assay of active ingredient content and yield of ZY-16 and ZY-29 which are derived from Liangshan Prefecture are higher than others in the collected germplasm resources, indicating that local seeds have a stronger adaptation to the environment; (4) considering the active ingredient content and yield of various germplasm resources, we screened out 4 copies YMC and 5 copies XAYMC as the original material for breeding.