The Regulatory Role For Non-coding RNA In Maize Row Number

Maize is one of the world’s three major food crops, and it is also an important economic and forage crops. Yield trait of maize is a complex quantitative trait, and subject to the combined effects of multiple traits, Therefore, to clarify the genetic mechanism of the maize yield and component factors will play an important role on the breeding of fine varieties of maize. Factor of three main components of the maize yield in different varieties of kernel per row and 100-kernel weight by the influence of outside environmental conditions, and row number per ear by ear development, the higher heritability and less susceptible to external environmental conditions play an important role to ensure that maize yields.miRNAs are a class of non-protein coding sequence length of 18-24 nt endogenous small RNA molecules, play an important role in the process of plant growth and development and adversity stress response. Therefore, to find the miRNAs about row number per ear, reveal the role of miRNA regulation of row number per ear initially,which is important to know the genetic mechanisms of the maize row number per ear.Group of preliminary studies on the basis of a Zong 3 and Zong 3 SSL(single segment substitution lines, SSL-10) materials, get the miRNAs about row number per ear and its target genes by high-throughput sequencing(Solexa) technologies and bioinformatics methods, analysis miRNA and target gene expression of row number per ear by real-time quantitative PCR. The main results are as follows:1. Reads of about one million small RNA by Solexa sequencing, compared with the miRNA database. Ear developmental stages of the two samples detected 145 and 144 known miRNA.145 and 144 known miRNA belonged to 17 miRNA families, and were conserved among rice, Arabidopsis and other species.2.168 novel candidate miRNAs were detected in maize ear by deeping sequencing.68 candidate miRNAs have further confirmed.that these 68 candidate miRNA in the two samples were compared to 31 novel miRNA.3. For the 85 known miRNAs,158 potential targets were predicted. A small number of miRNA did not find the corresponding target gene, a portion of the miRNA target genes are multiple but belongs to a class, part of the miRNA target genes is more and belong to more than one type. These target genes involved in transcription and translation, plant photosynthesis, stress, signal transduction.4. Through data processing, fluorescent quantitative confirmation, as well as its target gene prediction analysis.Differentially expressed of miRNAs in Zong3 and Zong3 SSSLs(SSL-10), miRNA regulation of its target genes affect maize row number per ear finally.