Effect Of Interaction Of Gln And IMO On Immune Functions And Small Intestine Function In Weaned Piglets

The purpose of the thesis was to study the influences of Glutamine(Gln) and Isomaltooligosaccharide(IMO) on immune function and intestinal function. Eighty-one weanling pigs(Duroc×Landrace×Large white, 8.12±0.47 kg average body weight) of the similar age in days were selected. It were divided into nine treatments with three replicates of three, a control group was not added Gln and IMO. Experimental groups were fed with different doses of Gln and IMO in the dietary. Preliminary feeding period of this test is seven days, Trial period is 28 th days.1 Adding Gln(10g/kg or 15g/kg)*IMO(10g/kg) significantly increased weaning daily gain and daily feed intake. Adding Gln(10g/kg)*IMO(10g/kg) are more significant improvement in growth performance in weaned piglets. Adding(10g/kg)*IMO(10g/kg) were significantly reduced serum BUN and TCH concentration in weaning pig. Gln(15g/kg)*IMO(6g/kg) increased significantly the weaning of serum AKP concentration. While reducing each test group piglets weaned MDA concentration in serum.2 Significantly improve the interaction between the two tests for 7d and 14 d weaned serum IgG and IgM concentrations, added separately Gln(10g/kg) were significantly increase testing for 7d and 14 d weaned serum IgA concentrations. Gln(10g/kg)*IMO(10g/kg) were significantly increase testing for 7d and 28 d weaned serum albumin concentration. Gln(10g/kg)*IMO(6g/kg) were significantly increase testing for 7d and 14 d piglets weaned T-AOC concentration in serum. Gln(15g/kg)*IMO(6g/kg) were significantly increase the experiment groups weaned serum SOD concentration, while reducing each test group piglets weaned MDA concentration in serum.3 Adding Gln(10g/kg or 15g/kg) and IMO(10g/kg) in the diet could significantly improve the number of lactic acid bacteria and bifidobacteria, reducing the number of E. coli in cecum, jejunum and ileum contents in weaned piglets. Adding in the diet Gln(10g/kg)*IMO(10g/kg) significantly improved weaning piglets duodenum, jejunum villus height and villus width, can effectively reduce piglet duodenum, jejunum crypt depth.4 Gln and IMO interaction group SGLTl and GLUT2 mRNA expression was higher than the control group and a separate add IMO group. Individually adding IMO could improve duodenum and ileum mucosa SGLTl and GLUT2 mRNA expression levels, while reducing SGLTl in piglet jejunum mucosa and GLUT2 mRNA expression.