| Two experiments was performed to research the Metabolism, Deposition and Residue Depletion of Phenethanolamine A in Swine, aim to provide scientific data for appropriate regulation of this illegal drug.Exp.l Study on Metabolites and Metabolic Regularity of Phenylethanolamine A in SwineThis study aim to investigate the metabolic mechanism of Phenylethanolamine A in swine by measured the metabolic product in blood, urine and feces when feed diet with Phenylethanolamine A. Nine DLY pigs (55kg body weight) were randomly distributed to 2 treatment:control group (three replications, each with one pig) and experimental group (six replications, each with one pig). On the first day of experiment, the experimental group were feeding diet contained Phenylethanolamine A (20mg/kg) for 20min ad libitum (average 24.08±1.71 mg Phenylethanolamine A for each pig), then changed to basal diet; the control group were fed basal diet all the time. At the time of 0.5,1,2,4,6,12 and 24 hours after feeding,20mL blood was collected from jugular vein each pig (six pigs in experiment group, three pigs in control group); Feces and urine was collected every 4h during the 48h experiment. Phenylethanolamine A in blood, urine and feces was analyzed with high performance liquid chromatography tandem mass spectrometry, and the metabolic product of Phenylethanolamine A in urine was analyzed with time-of-flight mass spectrometry.The result showed that Phenylethanolamine A can be detected in blood 0.5 hours after feeding, and get the largest amount at the time of 2 hours after feeding (12.28±1.36ng/mL); half-life period in blood was 2.07±1.38h. The largest amount of Phenylethanolamine A in urine appeared 8h after feeding (282.68±124.74ng/mL); and the content of Phenylethanolamine A in urine was above 100ng/mL during 16h after feeding. The amount of Phenylethanolamine A in feces showed an increased trend during 0 to 48h, and reaching the largest amount at 32h after feeding (62.18±8.11ng/g). Within the 48h experiment, Phenylethanolamine A excreted from urine was greater than fecal. There were 9 kinds of Phenylethanolamine A metabolites detected in urine through the one-stage flight mass spectrometry; and 7 kinds of metabolites were confirmed through the two-stage flight mass spectrometry.Exp.2 Study on the Deposition and Residue Depletion of Phenethanolamine A in SwineThis study aimed to research the deposition and residual depletion of Phenethanolamine A in tissue and succus of Swine. Thirty-four DLY pigs (67.33±2.80kg) were randomLy distributed to 2 treatments:control group (ten replications, each with one pig) and experimental group (twenty-four replications, each with one pig). The control group was feeding with basal diet (without Phenethanolamine A) all along the 42 days experiment. The experimental group was feeding with diet contained Phenylethanolamine A (20mg/kg) during the starter of 28 days, and then change to basal diet during the finisher of 29 to 42 days. During the trial period, water and feed were provided ad libitum. At 11:00 o’clock(3 h after administration) on the 1,3,5,7,9 and 11 days of the experiment, six pigs in experiment group and three pigs in control group were chosen for blood collecting from jugular vein (20mL). Six pigs in experiment group and two pigs in control group were slaughtered for sample collecting on 15 and 29 days of the trial. When the urine testing (Phenylethanolamine A dipstick) of experimental group was negative (three days after the diet changed to basal diet in our experiment), and at 36 and 43 days four pigs in experiment group and two pigs in control group were slaughtered for sample collecting. Samples were determined by High Performance Liquid Chromatography Tandem Mass Spectrometry.The result showed that Phenylethanolamine A in blood accumulated with time, and reached a dynamic equilibrium at 7～11 day of the trial (20.77～24.31 ng/mL). When feeding with diet contained Phenylethanolamine A (20mg/kg) for 14 days, the deposition of Phenethanolamine A in tissues and humoral of Swine were:Lung 305.86±195.2, Kidney 12.19±7.21, Liver 4.11±1.73, Muscle 4.26±1.87, Blood 0.85±0.46, Urine 50.26±22.32,When feeding with diet contained Phenylethanolamine A (20mg/kg) for 28 days, the deposition of Phenethanolamine A in tissues and humoral of Swine were:Lung 95.53±33.78, Kidney 2.67±1.28, Liver 0.97±0.25, Muscle 2.26±0.41, Blood 0.91±0.25, Urine 17.33±8.24, Phenethanola- ine A couldn’t be detected in blood 7 days after the diet changed from experimental diet (Phenethanolamine A contained) to basal diet. Phenethanolaine A in kidney, muscle, liver and urine couldn’t be detected 14 days after the diet changed. But it was still distributed in lung at the end of the trial. |
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