Reseach Of Granule Formulation For Streptomyces Purpeofuscus

Streptomyces purpeofuscus YSSPG3 was isolated from rhizosphere of healthy hybrid bamboo in Sichuan, which has showed strong inhibiting in the suppressing of Arthrinium phaeospermum and had certain development potential application in biological control of plant diseases. At present, using of Streptomyces purpeofuscus prevention to control these plant diseases was underreported both in domestic and abroad. By using liquid and solid two-phase fermentation process, optimized solid state fermentation conditions of Streptomyces purpeofuscus YSSPG3, screened out suitable carrier and adhesion agent, which had improved the containing condition of YSSPG3. At the same time, we developed it into granule and measured its growth-promoting effect and disease prevention, which were better to its future application, providing the theoretical basis for the development of biocontrol agent YSSPG3. The result of this study as follows:1、The optimal medium for seed liquid was as follows:Glycerol 0.5 g/L, Dextrin 0.5 g/L, Soybean powder 0.5 g/L, Ammonium oxalate 1g/L, CaCO3 0.3 g/L, NaCl 0.2 g/L, pH 7, loading amount 100 mL/300mL, inoculum 2%, rotating speed 140 r/min, temperature 27℃, the seed age 4 d. In this condition, the number of living bacteria and antibacterial rate was higher.2、Optimized the solid-state fermentation condition of YSSPG3 was as follow:corn grit 10 g, soybean powder 13 g, oluble starch 5 g, rice bran 8 g, wheat bran 8 g, soybean meal 4 g, pH 7, inoculum levels 20%, initial moisture content 50%, temperature 27 ℃,the seed age 9 d, turning at 12 h. Under the optimized condition, the alive cells amount and antibacterial rate will reach the best.3、0.3% CMC-Na was added as a binder, plant as was added as a coating agent, which were unfolded on the kraft paper, air-dryed at room temperature with airflow, After drying is finished as the YSSPG3 granules agent. When YSSPG3 granules was stored in the dark at room temperature and 4℃ refrigerator, the survival rate was significantly higher than that of fermentation products without any treatment. In these two kinds of storing modes,Stored at 4℃ in the fridge was the most stable, the shelf life of at least 6 months, the survival rate was 76.39%.4、Measured at the rate of 3g/basin, the control effect of Sclerotinia sclerotiorum was the best, the effects of controlling diseases were 74.36%. The application of the agent had no effect on the growth of Gerbera jamesonii seedling, and there was a certain growth promoting effect on plants.