Toxicity Effects Of Composite Amanita Virosa Lam. : Fr.’s Toxins Crude Products To The Pyrrhalta Aenescens(Fairmaire)

Pyrrhalta aenescens(Fairmaire) is a serious pest of elm, it is very common in northeast and north of China. Adults and larvae are feed by leaf, often eat the whole plant elm leaves,leaving only the vein. The authors collected and recognized the parts of large fungi and plant in Liaoning Province, extract the both effective components, and insecticidal tests were carried out on Pyrrhalta aenescens(Fairmaire) adults. The main results were listed as follows:1. Extractions of 41 speciescollected macrofungi were used to testpoison effect on P.aenescens(Fairmaire), the stabilityof macrofungi extraction was test,which correction mortality rate achieved100% in 24 h. Extractions from two kinds of large fungi were selected, which are the species of Lepiota cristata(Bolt. : Fr.) Quél.and Amanita virosa Lam. : Fr.. insect poison effect of A.virosa Lam. : Fr. extraction was better.2. Extractions of 46 species collected plantwere used to testpoison effect on P.aenescens(Fairmaire) adults, the stability of plant extraction was test,which correction mortality rate achieved 100% in 24 h. Extractions from three kinds of plant were selected,which are the species of Pulsatilla chinensis(bunge)Regel, Platycodon grandiflorus(Jacq.)A. DC. and Taraxacum platypecidum Diels. insect poison effect of T. platypecidum Diels ethanol extract was better.3. The selected two species of macrofungi and three species of plant were matched according to the quality of 100:1. Six kinds of complex biological extractions were respectively used for P. aenescens(Fairmaire) poisoning experiment. The results show complexes of A.virosa Lam. : Fr. and T. platypecidum Diels on insect toxic effect were obviously enhance. The cotoxicity coefficient reached 117.460, therefore, this compound was selected.4. Complex biological extractions were final selected for safety detection of zebrafish.LC50 of complex biological extractions to zebrafish was minimum in 72 h. According to《Biological detection technology specification(water environment part)》standard,the toxicity of this compound is low.5. The insecticidal test of final selection composite biological extractions was done in field. and the 1000 times of the CyperMethrin and sterile water were treat as control group. It was found that the extraction of this complex was affected by the weather, and the effect of killing is less than 1000 times CyperMethrin.The purpose of this experiment is study on macrofungi and plant extractions, and select the type, which can control the P. aenescens(Fairmaire). Provide a new way to find safe and effective environmental friendly biological pesticides.