Variation Of Strains Of Soybean Mosaic Virus In Heilongjiang Province In China

Heilongjiang is the largest area of planted soybean provinces, soybean mosaic virus (Soybean Mosaic Virus, SMV) is a worldwide disease of soybean diseases, serious impact on yield and quality. SMV strains differ divided vary widely at home and abroad, and domestic researchers have adopted their own set of differential hosts, leading to differences in the division of SMV strains, which is not easy to communicate between each other. Therefore, a national unity SMV strain identification system was established, include Nannong 1138-2, Youbian 30,8101, Tiefeng 25, Davis, Buffalo, Zaoshu 18, Kwanggyo, Qihuang No.1, Kefeng No.l 10 varieties. Under this identification system, the distribution and composition of SMV strain had been reported in main soybean producing areas of northeast China, the Huang-Huai and southern soybean growing areas. There had been reported total twenty two strains SC1 to SC22.For the issues such as SMV strains identified the passage of time and a small number of virus samples in Heilongjiang province,to collect leaves with typical symptoms of SMV extensive acquisition in field plots,farm and experimental field in Harbin City, Suihua City, Bei’an, Wudalianchi City, Qiqihae City, Mudanjiang City and Jiusan farm field in Heilongjiang Province, and then after the initial breeding identification, separation and purification, serology, using the same set of identification system, the positive isolates were identified so we can clearly know the composition and distribution of SMV strains in Heilongjiang region so far, and compared with the past identification results compared to know the dynamics change of SMV strains in the region. The main results are as follows:1. The identification and distribution of SMV strains in Heilongjiang Province in ChinaIn July 2012 soybean flowering peak, production field and experimental fields of breeding units in Heilongjiang Province, typical symptoms leaves of SMV were widely collected total 461 samples. After preliminary breed identification, serological testing, a single plaque purification and separation serological identification, get 63 SMV isolates. With the 10 differential host varieties used before,63 isolates were identified. According to isolate anti- sense reaction on 10 differential hosts will return to the 2 groups.6 which is a group classified as SC15 strain, SC18 strain classified as rest. SC15 could system infection 10 differential hosts; SC18 could only system infection Nannong 1138-2 and 8101, SC15 and SC18 were each 9.5% and 90.5% of the total.2. The variation of SMV strains in Heilongjiang ProvinceCompared the strains identified in this study with the three strain groups reported by Lv,SC18 is similar to its No.1 strain group,SC15 is similar to its 3rd strain group in susceptible range. And the results were compared with Wang et al, found between the main strain SC18 identified in this study and their main strains SC11 only differences in the Tiefeng 25, SC18 can not infect Tiefeng 25.