Research Of The Populations Composition And Controlling Technologies On Root Knot Nematode In Pretected Vegetable In Xinjiang

Root knot nematode disease has occurred more and more frequently in xingjiang greenhouse vegetables in recent years. So we have conducted an investigation on the occurrence of vegetables root-knot nematode diseases in this study. Using molecular biology methods combined with morphological to identify nematode species and carried out safety prevention and control technology studies on root-knot nematode disease, the main results are as follows:After our investigation on root-knot nematode diseases in south and north of xinjiang, we found that root-knot nematodes has been occurred and distributed only in greenhouse vegetables in Hetian、Akzo、Korla、Turpan、Yili,but could not found in reveals.Fourteen samples of root-knot nematode were collected in the greenhouse planted with various vegetables from the occurred places. Most of the host plants were tomatoes, and cowpea、cucumber、lettuce、celery、bitter melon and so on.By adopting morphology and root-knot nematode-specific primers PCR methods, we could identify the populations. The results indicated that samples of knot-root nematode belong to M.incognita and M.hapla. The dominant populations was M. incognita.We have used the sick soil inoculation method to screen the resistant vegetable varieties from 26 varieties. The results showed that among the nine pepper varieties, jinla 0903, texuansipintou, jufeng NO.1, tedaniujiaojiao, guota 106 and jingla 8 hao were immune varieties. Among the six tomato varieties, Dior, Phoenix was resistant varieties. Among the eight cucumber cultivars, xinyaniv, Jin mei fung on the 3rd, Jin shuai No.1 and meiyu were low-resistant varieties. Damazi bitter were highly resistant varieties, Thai oil green gourd and cowpea were resistant varieties.The results of greenhouse vegetable root knot nematode in xinjiang safety prevention preliminary study were as follows:Using the sprout that had been treated by hot water to perform the indoor pot culture disinsectization experiment. It showed that when last 15 min at 48℃, prevention index was 67% and plant survival rate was 90%. When the condition became hold on 30 min at 48℃, prevention index was 1, the plant survival rate was 85%, when the temperature exceeds 50℃, the prevention index was 1, however, the plant survival rate generally less than 70%.Taking advantage of the unique photo-thermal conditions in xinjiang and combined with the calescence prevention and cure experiment, we could make these conclusions: the most effective treatment combination was: depth of 20 cm, watering, add cow dung and the most effective prevention and cure combination was the with cover one, up to 85.7%.Five kinds of pesticides was tested by impregnation method on inhibition rate of root knot nematode egg masses, the results showed that guizhou sinochem 5% abamectin had the highest sacs inhibiting rate, and the effect of the biological bacterium agent was the worst.Using natural high temperature combined with pesticides prevention to prevent root knot nematode, the results showed that in the depth of the soil layer 20 cm, fosthiazate showed more prevent effect index among seven kinds of pesticides, followed by genjiening.We used normal temperature pot method to detect 7 kinds of pesticide effect in root knot nematode prevent. The results showed that guizhou sinochem 5% abamectin and fosthiazate prevent effect was best, followed by tianjin hanbang 5% abamectin and genjiening, ningdun and shudekang biological bacterium agent effect was relatively low.The field of greenhouse experiment results showed that compound method of prevention and cure with fosthiazate and genjiening combined with mulching and cow dung was best, the medication was 2.0 kg/mg prevent effect reaches 100%, then 5% abamectin, prevent effect of 90%.