The Proliferation Of Endosperm Callus Of Aronia Melanocarpa And Antioxidant Activity

Aronia melanocarpa Elliot, which belongs to Rosaceae, Sorbus, is regarded as an excellent species of deciduous flower shrubs as its affluent and edible nutriention、 highly official value、ornamental value and ecological value. The domestic research on Aronia melanocarpa has gradually improved but reports concerning polyploidy breeding. Therefore, we used the mature endosperm as experimental material for the sake of the influential factors concerning about callus proliferation to lay the foundation for the regeneration of triploid plant and determined the antioxidant content of endosperm callus which were cultured in order to provide more abundant raw material for edible, medicinal and cosmetic processing and production.The researches on the factors influencing on proliferation of endosperm callus of Aronia melanocarpa Elliot indicated that the callus was compact and the induction rate was highest when the sampling time was colour-changed stage; When the endosperm callus inoculated on MS medium, the structure was compact with a milky appearance which was higher than other treatments; sucrose was the best one for the callus induction and the sugar was the second which were both obviously higher than other treatments. The sucrose gave the highest proliferation coefficient 3.20 with the concentration of 40 g·L-1; There was no significant difference between 0 lx and 1600 lx, while the best illumination intensity was 800 lx; Different plant growth regulator combination had a great influence on the endosperm callus induction, the combination with TDZ and IBA gave the highest endosperm callus induction rate; The combination with BA and 2,4-D was little less but with a water-soaking structure; When the concentration of TDZ was 0.05 mg·L-1, the fresh weight was obviously higher than other treatments with a compact structure; When the concentration of IBA was 2.0 mg·L-1, the highest proliferation coefficient was 3.46; The fresh weight of endosperm increased firstly and then decreased with increasing temperatue from 15℃ to 30 ℃, when the temperature was 20-25 ℃, the fresh weight was obviously higher than other treatment. When the endosperm inoculated on MS medium added with TDZ 0.05 mg·L-1, IBA 2.0 mg·L-1 and sucrose 40 g-L-1 gave the highest proliferation coefficient when the illumination intensity was 800 lx and the temperature was 20 ℃. The fresh weight was 899.1 mg and the proliferation coefficient was 3.49 while the callus look yellow green with a compact structure.We used colorimetry method to measure the antioxidant content in order to compare endosperm callus with fruit, the results showed that the anthocyanin content of the endosperm callus was 0.24%, total polyphenol content was 2.33% and flavonoid content was 0.39%, they were lower and close to the anthocyanin, polyphenol and flavonoid content of fruit respectively. Therefore we could use endosperm callus as raw material for edible, medicinal and cosmetic processing and production to promote the research and development of relative products of A. melanocarpa.Determination of DPPH may explain the antioxidant capacity of endosperm callus and callus of leaves, cleaning up free radicals. Experiments showed that with the endosperm callus pigment concentrations increase, its antioxidant capacity have been increased. The antioxidant capacity of endosperm callus was better than callus of leaves.