Quality Evaluation Study On Cultivated Astragali Radix From Different Areas

Objective: To compare the chemical quality of different cultivars Astragalus herbs; To establish the chemical fingerprints and biological fingerprints on ASTRAGALI RADIX.Methods: 12 kinds of cultivated Radix Astragali collected from 9 different areas, planted in the same blocktest Tanaka, soil, climate, water and fertilizer,field management and so on.The samples were identified and determination of content inaccor-dance with the 2010 edition of Chinese Pharmacopoeia;Using single factor method and orthogonal experiment method,optimize extraction process of Astragalus polysaccharide, and determinate the content of polysaccharide in samples; By using the grey correlation analysis method to evaluate the chemical quality of different cultivated varieties of Radix Astragali; Established the biological fingerprints by protein polyacrylamide gel electrophoresis(PAGE), ultraviolet spectroscopy(UV) and High performance liquid chromatography(HPLC).Study on the genetic diversity of Radix Radix Astragalus by random amplified polymorphic DNA(RAPD).Results: The optimal extraction conditions of polysaccharides in Radix Astragalus: ratio of material to liquid 1:20, extracting for 3 times,50 min each time,the concentration of alcohol 70%.The order of chemical quality of Astragali Radix:Chifeng Mongolia >Bo zhou Anhui>Baoding Hebei>Shangluo Shanxi>Anguo A>Anguo B>Tangshan Hebei>Yixian Liaoning>Anguo C>Anguo D>Luonan Shanxi>Lushi Henan,correlation of the first three were higher than 0.50.The UV spectra of four kinds of extraction solvent of ASTRAGALI RADIX were established. The results showed that UV spectra of ASTRAGALI RADIX from different regions were very close, had the common characteristic peaks, showed that the main component was the same or similar. The HPLC fingerprint with Calycosin-7-O-β-D-glucoside as internal tandard reference peak of ASTRAGALI RADIX was established. 17 common characteristic peaks were determined, and the common peaks area accounted for more than90% of the total area. The PAGE fingerprint of ASTRAGALI RADIX was set up,12 common bands were determined. Using PCR-RAPD technology, 3 pairs of random primers were selected for the analysis of polymorphism of 18 pairs. 27 bands were obtained by PCR., and polymorphic bands was 26, the percentage of polymorphic bands was 96.3%.To a certain extent, the 12 batches of the tested medicinal materials have the abundant genetic diversity.Conclusion: The indicators of medicinal materials are in line with the Pharmacopoeia.The content of chemical composition in different samples are quite different.Astragalus which introduction form Anhui Bozhou,Chifeng Inner Mongolia,Hebei Baoding is better in chemical quality,and these should have more comprehensive variety comparison test.12 batches of Astragali Radix with abundant genetic diversity. The thesis has preliminary established HPLC-UV fingerprint,ultraviolet spectral line group and PAGE fingerprint, which can provide scientific basis for the identification and quality control of Astragali Radix.