Study Of TCM Prescriptions On The Prophylaxis And Therapy Of Dairy Cow Mastitis

Cow mastitis is among the furthest frequent and susceptible disease in the field of global dairy farming. Due to the abundant employment of antibiotics permanently, drug resistant strains is increasing and drug residue problem is severe, which threaten and affect mankind’s health. So the development of a safe and resultful medicine is imminent. The Chinese herbal medicine for precaution and cure of dairy cattle mastitis has its specific superiority, and can cover the shortage of antibiotics. Therefore, it is a up-to-the-moment projects that triturating a king of compound preparation of TCM for precaution and cure of mastitis.In tests,14 milk samples of cattle with mastitis of dairy farms were gathered in Qixian.8 strains of E.coli (25.8%),12 strains of S.aureus (38.7%) and 11 strains of Streptococcus (35.5%, mainly for S.agalactiae is) in 31 strains of pathogen were separated from 14 milk samples. The results illustrated that the dominating pathogens of cattle mastitis in this area were E.coli, S.aureus and Streptococcus (S.agalactiae is maximum). Drug sensitivity test datas said that in dairy farms, the susceptibility of E.coli to cefotaxime, ceftriaxone, cefixime, Amikacin and fosfomycin was high, but E.coli was resistant to most antibiotics; In addition, S.aureus and Streptococcus was moderate sensitive to the substantially all of antibiotics.In the test, according to the recessive mastitis disorders, syndrome differentiation TCM theory and fundamentals of TCM prescription, we formulate two Chinese medicine compound in different ideas. The main function of compound 1 is strengthening spleen and invigorating Qi, blood-activating and stasis-dissolving, and dredging collateral for promoting lactation. In addition, the leading function of compound 2 is clearing heat, purging fire, detonicating, activating blood and removing stasis. Through the antibacterial test in vitro and clinical trials, the findings cleared that in the trials group 1, the activity of ALP and LDH decreased after administration in whey and serum, but the downward trend were not obvious,while, NAGase activity increased after decreasing. In the trials group 2, the activity of LDH, ALP, and NAGase had a certain decline after administration in whey and serum. This indicated that in the cure of recessive mastitis, the compound one had a better effective function than the compound two.In the test, according to the upper experimental result for prescription of TCM thinking, clinical mastitis symptoms, syndrome differentiation TCM theory and fundamentals of TCM prescription, we formulate a herbal compound whose leading function of compound 3 is clearing heat antitoxicant, reducing swelling and dredging collateral for promoting lactation. Through the antibacterial test in vitro and clinical trials, it turned out that after treated, the activity of LDH, NAG in whey and serum declined very significantly(P<0.01); the activity of ALP in whey decreased marked-ly(P<0.05) and it in serum descend very markedly(P<0.01). This displayed that in the cure of clinical mastitis, the compound three had a marked therapeutic effect.