Stem Tip Culture Of Pugionium Cornutum

Taking stem tip of aseptic seed-derived plantlets as explants,tip meristem culture of Pugionium cornutum was establishing by apical meristem culture and axillary bud multiplication.The effects of explants size、light intensity on meristem culture were studied; The effects of medium、plant growth regulator on shoot inducement were studied in the process of primary culture; The effects of medium、type and concentration of plant growth regulator、AgNO3 concentration、sugar concentration and agar concentration on shoot multiplication were studied in the process of subculture,Taking the field of axillary bud as explants,the proliferation and growth situation was explored;The effects of medium、type and concentration of Auxin on plantlets rooting.The results were as follows:1、Stem tip culture:(1)The rate survival of bigger stem was higher;(2)The optimum light intensity for culturing apical meristem of P.cornutum was 36001x.2、Primary culture:(1)The optimum medium for inducting axillary was B5; Average induction axillary number was 3.37;(2)Stem segments explants were cultured on medium supplemented with 5.0mg·L-1 6-BA and 0.5mg·L-1、1.0mg·L-1 IBA, induction axillary number was 4.17、4.37.3、Subculture:(1)The optimum basic culture medium of all was MS,proliferation times reached 5.27;(2)The type of Cytokinin was 6-BA and concentration was 3.0mg·L-1 which was the optimum type and concentration of Cytokinin in this study;(3)The type of Auxin was IBA and concentration was 0.1mg·L-1, which was the optimum type and concentration of Auxin in this study;(4)The highest axillary bud proliferation 7.43 times was obtained on the medium of MS+6-BA3.0mg·L-1+IBA0.1mg·L-1;(5)Proliferation times extremely decreased for AgNO3;(6)The optimum concentration of sugar was 3.0%;(7)The concentration of Agar was 0.4%、0.6%;(8)The field P.cornutum plants were not suitable for propagation disinfected by 0.1% HgCl2.4、Rooting culture:(1)1/2MS was the optimum basic plantlets rooting medium with 4.00% rooting rate;(2)The optimum type and concentration of Auxin was NAA1.0mg·L-1,the rate of rooting reached to 26.32%;(3)Different Processing Concentration and Time of NAA on Rooting of Asepsis plant was 100 mg·L-1、60min, the rate of rooting reached to 72.00%.