The Research On Forest Soil Microbial Biomass Carbon And Nitrogen And Microbial Characteristics In DaQing Mountain Inner Mongolia

Secondary forest in Inner Mongolia is an important part of China’s forest resources. It plays a very important role in eco-environment construction and biogeochemical cycles. Being important components of the forest ecosystem, soil microorganisms are an important connection between forest vegetation and soil nutrient systems. Soil microorganisms play an important role in the process of maintaining the balance of ecosystems. But so far the study on the dynamics of soil carbon and nitrogen and microbial communities of secondary forest in Inner Mongolia is poorly. In this study,11 types of soil samples were collected on spring and autumn in DaQing Mountain, Inner Mongolia. The soil samples were analyzed by the Chloroform fumigation extraction method (FE) and Real-time fluorescence quantitative PCR. The results were as follows:The results showed that the soil microbial biomass, the ITS gene copy numbers and the 16S rRNA gene copy numbers were different under different rhizosphere. In spring, the microbial biomass carbon contents were 360mg/kg-984mg/kg. The microbial biomass nitrogen contents were 63mg/kg-178mg/kg. Bacterial 16S rRNA gene copy numbers were 8.8×108-3.6×109 copy numbers per gram of dry soil. The number of fungi ITS gene copies were 8.1×107-6.3×108 copy numbers per gram of dry soil. In autumn, the microbial biomass carbon contents were 818mg/kg-1342mg/kg. The microbial biomass nitrogen contents were 217mg/kg-240mg/kg. Bacterial 16S rRNA gene copy numbers were 1.7×109-4.1×109 per gram dry soil and fungi ITS gene copy numbers were 5.8×108-1.7×109 per gram dry soil. Based on the results, the influence of season and tree species on soil microbial biomass carbon and nitrogen and microbial abundance was significant. And soil microbial biomass carbon and nitrogen was positive correlated with soil microbial abundance.