Study On The Interaction Model Of LFY And UFO Homologous Gene In Pinus Tabuliformis

LEAFY (LFY) gene is a flower meristem identity gene. In angiosperms, there are many studies about LFY. However, the research about the two LFY genes in gymnosperms is less and need to be increased. Pinus tabulaeformis, a gymnosperms’ plant, was selected in our study. The interaction relationship of PtLFY and PtNEEDLY (PtNLY) genes, as well as PtLFY / PtNLY and PtUFO1/PtUFO2 of Pinus tabulaeformis, were verified respectively both inside and outside the cell by yeast-two hybridization and BiFC methods. The PtLFY and PtNLY were transferred to Arabidopsis plants. The results of the study are as follows:1. Two proteins, PtLFY and PtNLY, can interact in P. tabuliformis, but there are differences in the intensity of interaction, which showed that they have a synergistic effect. However, their function may be not redundant. PtLFY and PtNLY are the homologous genes of LFY, were successfully cloned from P. tabulaeformis and were verified by yeast-two hybridization and BiFC methods. But the intensity of PtNLY self-interaction was stronger than PtLFY self-interaction.2. There was significant difference between the interaction models of two LFY-like proteins and two UFO-like proteins in P. tabulaeformis, PtNLY may be the main regulator in the expression of class B gene. PtUFOl and PtUFO2 genes, the homologous genes of UNUSUAL FLORAL ORGANS (UFO), were successfully cloned from P. tabulaeformis. The interaction relationship between PtNLY and PtUFO1/PtUFO2 was proved to be exist by yeast-two hybridization and BiFC methods, while no interaction between PtLFY and PtUFO1/PtUFO2 was exist. It shows that PtNLY gene was the main gene in controlling the expression of class B gene together with UFO-like gene.3. The success of the TO generation seeds of the 35S;:PtLFY and 35S::PtNLY were obtained, which was helpful to the follow-up experiments. Transgenic technology was used to transfer consecutive 35S promoter into Arabidopsis plants, then we got the TO generation seeds of transgenic plants of the 35S::PtLFY and 35S::PtNLY.