Experimental Study On Several Kinds Of Important Economic Plants Under Factory Cultivation

In order to primary studies on plant factory cultivation, the Sinojackia xylocarpa, Populus tomentosa and Toona sinensis were cultivated in this study. Firstly, the tender leaves (Sinojackia xylocarpa) were tissue cultivated with different collection time and different disinfected time. The results showed that the optimal time of explants collection for tender tissue culture was April. During this period, the contamination rate of microbe on the plant was the lowest. The optimal sterilizing treatment for the tender leaves was 75% Alcohol 30 s+0.1% HgCl2 1-5 min. Different disinfected time had a distinctly effect on physiology and biochemistry of the callus. The soluble sugar in callus inducted with 1 min and 3 min disinfected time were significantly higher (P<0.05) compared with 5 min disinfected time. The soluble protein in callus inducted with 1 min and 3 min disinfected time was significantly lower (P<0.05) than that in callus with 5 min disinfected time. Different sterilization time had no significant effect on the activity of peroxidase and superoxide dismutase. The callus inducted with 3 min and 5 min disinfected time had a stronger tolerance (P<0.05) of salt stress compared with the callus inducted with 1 min disinfected time. Secondly, the tender stems of Populus tomentosa were used as the material to study the effects of different medium on the induction of callus, adventitious buds and roots. In order to promote the regeneration of Populus tomentosa, A medium (MS+0.1mg/L6-BA+0.1mg/LTDZ+0.3mg/LNAA), D medium (1/2MS+0.05mg/LNAA+0.5mg/L6-BA) and G medium (1/2MS+0.3mg/LIBA) were suggested to induce the callus, adventitious buds and roots, respectively. Lastly, the whole stems were used to assess the effects of different cutting traits on bud emergence and early growth of the Chinese vegetable Toona sinensis. Results showed that cuttings derived from 1-year-old seedling stems displayed better morphological and physiological characteristics than from other seedling ages. Cuttings of newly obtained from 1-year-old fresh seedling stems and derived from four seasons were all sprouted well in two soilless cultivation methods (hydroponics and cuttage). Cuttings derived from apical and middle positions in spring and basal positions in autumn displayed better performance than the other treatments. Cuttings cultivated by cuttage exhibited better physiological characteristics than by hydroponics. We concluded that the cuttings of Chinese toon could be cultivated all year round for vegetable production. To obtain faster and more even stands of Chinese toon sprouts in plant factory, we recommended the use of cuttings from 1-year-old Chinese toon seedling stems, apical and middle parts from spring and basal positions from autumn, and use of cuttage method.