| Plant growth is greatly affected by environmental abiotic stresses, such as high salinity, drought. Plants would be damaged or even dead under abiotic stresses, if its tolerance to these stresses is much lower. Modern gene engineering has played a key role in improving tolerance to salt stress in plant. Suaeda salsa L. is a typical euhalophyte, which can grow well in saline land while most conventional plants can not survive. Salicornia europaea L. is a euhalophyte which seedlings grow best in 400 mmol/L salt concentration.Alfalfa (Medicago sativa L.) belongs to moderate salt-tolerant leguminous plant, which is one of the most important forage. It can only grow healthily in soils with a low or medium content of sodium salts. Therefore, breeding new salt-resistant varieties of alfalfa can increase both the utilization rates of saline lands and the yield of high quality forage, which could provide adequate material assurance for the development of animal husbandry.In this paper,1225 and 1257 Algonquin alfalfa seeds was obtained separately by the pollen-tube pathway method with the total DNA of Suaeda salsa L. and Salicornia europaea L.. The result of screenning these seeds with 225mM NaCl in medium showed that the 18 and 15 alfalfa seedlings introduced by the total DNA of Suaeda salsa L. and Salicornia europaea L. were obtained respectively. The 10 and 9 lines alfalfa reproductive seedlings which were introduced with the total DNA of Suaeda salsa L. and Salicornia europaea L. respectively were treated by salt stress, and the content of chlorophyll, malonedialdehyde, proline, soluble sugar, catalas activity in the leaves of these lines were investigated after salt treatment. The results show that 3 lines alfalfa transformed by total DNA of Suaeda salsa L. and 1 line alfalfa transformed by total DNA of Salicornia europaea L. reveal salt-tolerant physiological and biochemical properties compared with the control sample. |
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