| Soybean(Glycine max(L.) Merr.) as an important food and oil crop, not only contains rich protein and fat but also contains some nutrients such as isoflavone, oligosaccharides, which are good for human health. But in recent years soybean imports have increased year by year. Soyban can’t be self-sufficiency. Improving soybean yield and breeding good cultivars are the best way to solve the problem. Sibling cultivars Jidou17 and Ji nf58 as materials are elite soybean cultivars bred by crossing the maternal Hobbit line with the paternal line Zao5241. Their phenotypic traits, physiological, molecular markers and protein products were used to anlyze genetic basis of Jidou17, Ji nf58 and its parents, and to analyze the difference between Jidou17, Ji nf58 and its parents. The purpose of the paper was to provide certain theoretical guidance for breeding.The genetic backgrounds of Jidou17, Ji nf58, and their parents were analyzed by using 408 pairs of SSR primers. The result showed that the genetic contribution rates of the maternal line Hobbit and the paternal line Zao5241 to Jidou17 were 79.3% and 20.7%, respectively. The genetic contribution rates of Hobbit and Zao5241 to Ji nf58 were 58.9% and 41.1%, respectively. Genetic contribution of Hobbit to Jidou17 and Jinf58 was higher than Zao5241. Four cultivars were planted in field in continuous 2 years. The main agronomic traits including plant height, effective branch number, number of nodes on main stem, seed number per plant, seed weight per plant, 100-seed weight, and physiological indexes including photosynthetic rate, chlorophyll content, soluble protein content, soluble sugar content were got. The results showed that Jidou17 and Ji nf58 were higher than their parents in phenotype and physiological level. In addition, four cultivars to soybean mosaic virus SC3 had been identified. There had no significant difference on seed weight per plant, plant height and seed number per plant between check and SC3-inoculated plant, they inherited the advantages of their parents.A total of 1269 proteins were detected, in 14 days leaves of Jidou17 and its parents by i TRAQ method. Four proteins on the photosynthetic pathways and jasmonic acid synthesis pathway were studied, which were from differential protein between Jidou17 and its parents. They are chlorophyll A/B binding protein 1(CAB1) related to the original response, oxygen increased protein(OEE1), sedoheptulose-1, 7-2 phosphatase(SBPase) and lipoxygenase(LOX) related with electron transport. The corresponding monoclonal antibodies were prepared. Protein imprinting technology was used to detect the expression in four different periods of leaves, which were after seed emergence 2 week, 4 week, 6 week, 8 week, and three different periods of pods, which were after flowering 2 week, 3 week, 4 week, among sibling cultivars and their parents. The results showed that the accumulation expression levels of four proteins in four periods of leaves in Jidou17 were higher than his parents, in periods of pods higher than parents or one parents. Accumulation expression level of CAB1, SBPse, LOX protein expression in four periods of leaves and pods in Ji nf58 were lower than its parents, but OEE1 higher than parents, in periods of pods lower than parents, this was related to protein activity. Accumulation expression level of LOX protein on the jasmonic acid synthesis pathway in Jidou17 was higher than its parents, Ji nf58 lower than parents.Jasmonic acid synthesis pathway related to disease resistance. Jidou17 and Ji nf58 were resistant to SC3 and inherited the advantages of their parents.CAB1, OEE1, LOX, CATA1, SOD primers were designed. Gene expression of sibling cultivars and their parents were detected by RT-PCR technology, in order to study different expression between Jidou17, Ji nf58 and their parents.The results showed that there were different expression in different gene of different cultivars.Gene expression level of CAB1 in Jidou 17 and Ji nf58 was higher than Hobbit, lower than Zao5241, OEE1, LOX, CATA1 higher than their parents, SOD lower than their parents. Compared to protein expression level, gene expression level is different.Above all, through the genome-wide SSR markers, we tracked the pedigree of Jidou17 and Ji nf58 genetic fragments. Through the western blotting, we detected different expression of the protein of the Jidou17 and Ji nf58 with parents in the leaves and pods of different times. Through quantitative PCR, we analyzed gene expression differences between varieties. In summary, with Jidou17 and Ji nf58 agronomic traits, physiological indices and disease resistance, we established the phenotypic and molecular database of key varieties and provided more comprehensive theory for improving breeding varieties. |
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