Simultaneous Determination Of Residues Of Chloramphenicol, Ractopamine And Zearalanol With Its Analogs In Milk And Dairy Products By HPLC-MS/MS With Immunoaffinity Column

As concerns about food safety, the development of veterinary drug residue and thebanned drug detection technology also has attracted the attention of a great scientist. Thisstudy for the systematic research of chloramphenicol, ractopamine and zearalanol with itsanalogs in milk and dairy products and swine urine with immunoaffinity columncleanup-HPLC/MS/MS method. To obtain the main results are as follows:(1) An immunoaffinity cleanup-HPLC/MS/MS method was established for thesimultaneous determination of residues of chloramphenicol, ractopamine and zearalanol withits analogs (α-zeranol, β-zeranol, α-zearalenol, β-zearalenol, zearalanone and zearalenone) inmilk. The sample was cleaned up and enriched on an immunoaffinity column and detected byHPLC-UV. The recoveries of milk added with eight target compounds at different spikedlevels ranged from74%to101%, the limitation ranged from0.02μg/L to0.08μg/L and therelative standard deviations were less than7%. The sample was cleaned up and enriched onan immunoaffinity column and detected by HPLC-MS/MS with an electro spray ionization(ESI) source and multi-reactions monitoring (MRM) technology. The recoveries of milkadded with eight target compounds at different spiked levels ranged from71%to102%, thelimitation ranged from0.001μg/L to0.01μg/L and the relative standard deviations were lessthan6%.(2) An immunoaffinity cleanup-HPLC/MS/MS method was established for thesimultaneous determination of residues of chloramphenicol, ractopamine and zearalanol withits analogs in powder. The sample was cleaned up and enriched on an immunoaffinity columnand detected by HPLC-UV. The recoveries of powder added with target compounds atdifferent spiked levels ranged from69%to102%, the limitation ranged from0.10μg/kg to0.40μg/kg and the relative standard deviations were less than6%. The sample was cleaned upand enriched on an immunoaffinity column and detected by HPLC-MS/MS with an electrospray ionization (ESI) source and multi-reactions monitoring (MRM) technology. The recoveries of powder added with target compounds at different spiked levels ranged from64%to99%, the limitation ranged from0.005μg/kg to0.05μg/kg and the relative standarddeviations were less than5%.(3) An immunoaffinity cleanup-HPLC/MS/MS method was established for thesimultaneous determination of residues of chloramphenicol, ractopamine and zearalanol withits analogs in swine urine. The sample was cleaned up and enriched on an immunoaffinitycolumn and detected by HPLC-UV. The recoveries of swine urine added with targetcompounds at different spiked levels ranged from69%to99%, the limitation ranged from0.15μg/L to0.60μg/L and the relative standard deviations were less than6%. The samplewas cleaned up and enriched on an immunoaffinity column and detected by HPLC-MS/MSwith an electro spray ionization (ESI) source and multi-reactions monitoring (MRM)technology. The recoveries of swine urine added with target compounds at different spikedlevels ranged from60%to99%, the limitation ranged from0.008μg/L to0.08μg/L and therelative standard deviations were less than6%.