| The pre-sowing sugarcane stems were soaked by chitosanoligosaccharides (COS) solution (100mg/L) and ultra pure water for24hrespectively, then the stems were planted in plastic pots filled with sandyloam soil, respectively. After30days, the samples of leaves were taken.Next the sugarcane seedlings were treated by natural drought stress, thesamples of leaves were taken in the21th day.The results of physiological and biochemical indexes of sugarcanedisplay that COS can significantly improve sugarcane stem height anddiameter, and increase the content of chlorophyll a, chlorophyll b, totalchlorophyll in sugarcane leaves, meanwhile COS can significantly reducethe soluble sugar content in sugarcane leaves. Under drought stress, COStreatment can promote the drought resistance of sugarcane and increasethe content of chlorophyll b, total chlorophyll, soluble sugar, solubleprotein and leaf relative water content in sugarcane leaves, but themalondialdehyde content was reduced by COS treatment under droughtstress in sugarcane leaves..The proteins in sugarcane leaves were separated and identified usingproteomics methods based on two-dimensional electrophoresis and massspectrometry. The results showed that expression patterns of typicaldifferential proteins were consistent under COS treatment with thenormal water supply and the natural drought stress. Plastidic aspartateaminotransferase was down-regulated, while protein kinase, cytochromeb6/f complex protein (N, subunit) and oxygen-evolving enhancer protein2, isoflavone reductase-like, actin cross-linking protein, RNA helicasewere up-regulated.COS can stimulate the photosynthesis and improve the growth ofsugarcane by protein kinase, cytochrome b6/f complex protein (N,subunit) and oxygen-evolving enhancer protein2up-regulated and plastidic aspartate aminotransferase down-regulated. Under drought stress,COS can improve the expression of IRLs, Actin cross-linking protein andRNA helicase to increase the drought resistance of sugarcane. |
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