Research On Fluorescent Marker Of Lutjanus Erythopterus

The research object was focused on Lutjanus Erythopterus, one of important commercial fish in Beibu Gulf. Fishs were marked with chemical fluorescent marker technik. Some physical datas of ARS and calcium indexing have been studied. These results could provide theoretical basis and references for the species stock enhancement. The main research results are listed as follows:1、To study the marking techniques of Lutjanus Erythopterus with fluorescent marker, Fishs were immersed in different concentrations of Alizarin red S(ARS) and alizarin complexation(AC), compared with marking effect of the two reagents for Lutjanus Erythopterus. The results showed that:The dosage of ARS from20～600mg·L-1and AC from100～300mg·L-1were used to mark fishs, immersion for24～36h and18～36h. Fluorescent tags could be observed in the dorsal fin、fish scales and caudal fin of the fishes. Fluorescent marking effect is proportional to dosage of soaked liquid and the soaking time, but survival rate is inversely proportional to them, the mark rate of all marking groups were100%. Considering the factors such as the survival rate, the mark rate and the strength of the fluorescent marked effect, The optimum dosage of ARS and AC were300mg·L-1and200mg·L-1, the best soaking time was36h. Fluorescent tags can still be observed after breeding60d. There was no sinficant diffrence on survival and gowth of marked fish relative to controls throughout experiment.2、The related properties of dyeing agent in seawater were studied in this paper, The results showed that:The dissolved concentration of ARS in seawater was30mg·L-1; the main ingredients of precipitation in dyeing liquid was the ARS and calcium indexing; the dissolved content of ARS after remove sediment in100～300mg·L-1ARS seawater solution was about30mg·L-1.The dissolved concentration of CaCl2in100mg·L-1ARS was30mg·L-1.3、The properties of ARS and calcium indexing were studied by ultraviolet visible spectrophotometry. There had some characteristic absorption peak of ARS and calcium indexing at λ=507nm; the complex ratio of it was2:1, the form of it is Ca[ARS]2, Complex equilibrium constant K=5.0276x109.