Study On The Changes Of Serum Lipid Metabolism And Liver Function Related Indicators In Peripartum Dairy Cow

40prenatal45d cows have been chosen to study the dynamic changes of serous indicators of fat metabolism and liver function and the relevance between them in perinatal cow. Their whole blood was collected from the tail vein every5d to detect the concentration of serous Glucose (GLU), Triglyceride (TG), Cholesterol(CHOL),(3-hydroxybutyric acid (β-OHB), High density lipoprotein (HDL), Low density lipoprotein(LDL), Total protein (TP), Albumin (ALB) and activity of Aspartate aminotransferase (AST), Alanine aminotransferase (ALT), Alkaline phosphatase (ALP), y-glutamyl transferase (GGT) by automatic biochemical analyzer. The concentration of serum Free fatty acids (NEFA), Apolipoprotein Al (apo-Al), Apolipoprotein B100(apo-B100), Leptin (Leptin), Neuropeptide Y (NPY) were detected by enzyme-linked immunosorbent assay. All results provided the theoretical information of the metabolic characteristics of the perinatal cows.The experimental results were as follows:the concentration of serous GLU, TG, CHOL, HDL, and LDL showed a trend of decline from the prenatal45d to the calving day (0d), and then increased gradually after childbirth. In addition, there were significant differences between different times (P<0.05). The concentration of serous NEFA, P-OHB, Leptin, and NPY were at a low level, and rose gradually until the calving day, thereafter fell sharply, except the NPY. In addition, there were significant differences (P<0.05) between different times. The concentration of serous apo A1and apo B100were at a high level on the prenatal45d, and then showed a declining trend. The activity of serous AST, ALP, GGT were at a certain level before prenatal10d, then increased significantly (P<0.05). The activity of serous AST and GGT showed a higher level after childbirth, serous ALP reached the peak in calving day (0d) and then decline sharply. The activity of serous ALT, TP and the concentration of serum ALB didn’t show an obvious change before the calving day. They showed a downward trend on the day of calving, thereafter rose significantly. In addition, there were significant differences (P<0.05) between different times. The correlation results of above indicators were as follows:there were a highly significant positive correlation (P<0.01) between fat metabolism indicators, GLU with NPY and TG with β-OHB, LDL, apo-Al, apo-B100, Leptin and CHOL with HDL, LDL and β-OHB with apo-Al, Leptin, respectively. There was a highly significant negative correlation (P<0.01) between NEFA and CHOL, HDL, LDL. There was a significant negative correlation (P<0.05), comparing GLU with apo-B100, and apo-Al, apo-B100with NPY, showed, respectively. There was a highly significant negative correlation (P<0.01), comparing TG, P-OHB, Leptin with AST, GGT, TP and NEFA with ALT, TP and apo-Al, apo-B100with GGT, respectively. There was a highly significant positive correlation (P<0.01), comparing TG, apo-Al, apo-B100with ALB and CHOL with ALT, GGT, TP and HDL, LDL with ALT, TP, respectively. There was a significant positive correlation (P<0.05) between HDL with GGT. There was a significant negative correlation (P<0.05), comparing apo-Al with AST, TP and apo-B100with AST and NPY with ALB, respectively. There was a significant positive correlation (P<0.01), comparing AST with GGT, and GGT with TP, respectively. Simultaneously, there was no significant correlation among the rest of the indicators (P>0.05). All results above indicated that there were disorder of the metabolism of carbohydrate and fat, slight damage of Liver function in perinatal cows. Meanwhile, these indicators showed complex relations between each other.