Effect Of Arbuscular Mycorrhizal Fungi On Maize Inbred Lines Of Different Symbiotic Efficiency

AMF (arbuscular mycorrhizal fungus) can improve soil and water status in plants. At the same time, it can establish symbiosis with the majority of plants and increase the absorption of water and mineral nutrients for crop, improve resistance to disease, drought and other stress. Therefore, Arbuscular mycorrhizal fungi can raise the survival rate of plants and promote the growth of crop, then increase the yield and quality of plants.Maize is one of the world’s three main grain crops, as well as the important grass crops. It has been playing more and more important role in people’s life with the rapid development of industry and animal husbandry and the speedy growth of the world population. Therefofe, it has not met the demand of people for high quality maize only rely on traditional breeding to improve the yield and quality of maize as well as enhancing their resistance so far. The interaction of arbuscular mycorrhizae and maize can help maize improve nitrogen fixation and utilization of phosphorus in the soil and other elements, then improve maize production.At present, researches on the promoting effect on maize by AMF have made some progress. There was no report on promoting effect on maize inbred lines because of different AMF symbiotic efficiency. Our previous studies showed that there were significant differences in symbiotic efficiency of different maize inbred lines with AMF, the symbiotic efficiency, ranged from0%to100%. Through the investigation of the effect on the maize growth with different symbiotic efficiency by AMF, we want to explore which of the symbiotic efficiency have the best growth promotion on maize. At the same time, we expect that the symbiotic efficiency and the production practice of maize can be made a combination which might contribute to explore a new path of breeding and found the theoretical foundation for using the arbuscular mycorrhizal symbiosis.Our laboratory has380maize inbred lines which have been sequenced,336of the inbred lines have been detected the symbiotic efficiency with AMF. The candidate genes, controlling the symbiotic efficiency of maize and AMF, have been identified by GWAS (Genome Wide Association Studies). Based on the previous researches, we selected maize inbred lines which have different symbiotic efficiency with AMF to investigate the influence of different symbiotic efficiency on growth promotion of maize after inoculation with AMF. In this study, we selected eight maize inbred lines with different symbiotic efficiency and make them grew in the field, inoculated arbuscular mycorrhizal fungi. The result showed that high symbiotic efficiency have obvious promotion effect on plant height, stem height and stem diameter in the jointing stage and booting stage, while low symbiotic efficiency almost no promotion effect on maize. Then we elected another eight maize inbred lines of different symbiotic efficiency and make them grow in the in the greenhouse, inoculated arbuscular mycorrhizal fungi. In the seedling stage, the result showed that high symbiotic efficiency have obvious promotion effect on plant height, shoot fresh weight, shoot dry weight growth, while low symbiotic efficiency almost no promotion effect on maize. At the same time, the results showed that the corn f tof there is no significant effect on growth index of the maize’s underground part, such as dry weight and fresh weight of root and so on. Together, the results show that AMF can promote the shoot growth of maize, and the promotion effect is closely related to symbiotic efficiency.