Study On Biosorption Of Water-soluble Nuclide Uranium(Ⅵ)by Typical Microbial Under The Radioactive Envrionment

Radioactive waste is a sensitive problem in today’s public concern.Uranium and uranium compound is one of biggest nuclide hazard in nuclear tests and nuclear accidents. Both of them have hazard of radioactive and chemical toxicity. In order to investigate biosorption and recycling for water-soluble nuclide uranium(VI) under the radioactive environment, explore the possibility of microorganisms biosorption for water-soluble nuclide uranium(VI) under the radioactive environment. This research paper select drectly three bacterial strains, including D.radiodurans, E.coli, S.cerevisiae.Takin living cell and dead cell as biosorbent respectively, developed a series of research about biosorption of water-soluble nuclide uranium(VI) under the radioactive environment.Acoording to studing biosorption of water-soluble nuclide uranium(VI) by three living cells, the results show that for three living cells, the biosorption optimum p H of water-soluble nuclide uranium(VI) is 5.0 and the biosorption optimum time of water-soluble nuclide uranium(VI) is 24 h. Meanwhile, with increasing initial concentration of uranium, biosorption efficiency presents nonlinear degradation, but biosorption capacity presents nonlinear rising.Acoording to studing irradiation damage of biological macromolecules and biosorption for water-soluble nuclide uranium(VI) under the radioactive environment by three strains, the results show that amio acids is easily caused serious damage by different doses of irradiation, biomacromolecule(such as bovine serum albumin) is easily caused little damage by different doses of irradiation. Under the radioactive environment, the biosorption optimum p H of water-soluble nuclide uranium(VI) is 5.0, and the biosorption optimum C0 of water-soluble nuclide uranium(VI) is 50mg/L and the biosorption optimum time of water-soluble nuclide uranium(VI) is 180 min. Compared with the irradiation,biosorption efficiency of water-soluble nuclide uranium(VI) is obviously reduced. And with different irradiation time, three strains releases different amount of free radicals under the culture condition.Acoording to studing group screening of cells and reduction process, the results show that microbial cells surface groups of biosorption for water-soluble nuclide uranium(VI) are primarily amide group and phosphate group. Besides, by way of a series of reduction processes, including enrichment and ashing, we can obtain enormous weight reduction ratio and volume reduction ratio.These works could provide the corresponding research foundation for subsequent solidification and recycling nuclide.In summary, the results indicate that it is possible that microorganisms could biosorp and enrich water-soluble nuclide uranium(VI) under the radioactive environment. And it could achieve efficient reduction and recycling nuclide.