The Research On Active Ingredient Extraction For Anti Cardiovascular Diseases From Dual Traditional Chinese Medicine Of Ginkgo Biloba And Gynostemma By Ultrafiltration

With the increasement of cardiovascular disease risk factors such as obesity, unreasonable diet, lack of physical activity, the incidence and mortality rates of cardiovascular disease have increased year by year. Because of the high morbidity, low cure rate and much co-morbidities, there are numerous difficulties in the prevention of cardiovascular disease. Traditional medicine and modern pharmacological researches have shown that the Chinese medicines for blood-activating and stasis-dissolving have unique advantages in treatment of cardiovascular diseases compared with western medicine, which have the priority of good effect, high activities and little side effect. Currently, the preparations of ginkgo leaves and gynostemmas including capsules, oral solution, injection and aerosol etc. have been widely used in clinical treatment of cardiovascular disease, respectively, but their mixture has reported rarely. In this thesis, the ultrafiltration method has been used to abstract active ingredient of the ginkgo leaves and gynostemmas by exploiting the characteristic of controllable molecular weight size, and a comparison has been made between the single Chinese herb and the mixture compound to make clear the changes of the active ingredient. In addition, we have tried to get more mixture compound to exert the synergistic action of the multi-component and multi-target. We expect that the present work can provide a theoretical basis on the preparation of high-effective cardiovascular drugs.Main works are as follows:1. The rough extracts as well as their mixture were obtained by extracting the ginkgo leaves and gynostemmas in ethanol water system.2. Purified the rough extracts by using the ultrafiltration equipment, and then concentrated the ultrafiltrate by using the method of reduced pressure concentration, finally, dried under vacuum at room temperature.3. In the study of ultrafiltration extraction technology, schemes of single factor test were carried out to investigate the influence of ethanol concentration, ultrafiltration pressure, ultrafiltration temperature and ultrafiltration time on the active ingredient of the ginkgo leaves and gynostemmas as well as their mixture.4. On the basis of this experimental results, through the designing of orthogonal experiments, the best technology conditions were determined.5. The spectrophotometric method was adopted to determine the contents of Ginkgo biloba extract, flavonoids, gynostemma pentaphyllum saponins in the extractive. In addition, the high performance liquid chromatography(HPLC) method was used to determine the contents of bilobalide.According to the results of this research:1.The best ultrafiltration extraction method for anti cardiovascular disease effective component of Ginkgo biloba and Gynostemma and mixed traditional Chinese medicine(TCM) for ethanol concentration was 50%, the ultrafiltration pressure was 9.0 bar, ultrafiltration temperature was 30℃;2.Under the best ultrafiltration extraction method, ginkgo biloba extract flavonoids content was 5.13%;gynostemma extract flavonoids content is 4.52%; ginkgo leaf and gynostemma flavonoid in the mixed traditional Chinese medicine(TCM) extracts were 6.35%; ginkgo biloba extract total lactone content is 0.3424 mg/g, ginkgo biloba and gynostemma total lactone content in the mixed traditional Chinese medicine extract were 0.3328 mg/g;Gynostemma extract saponin content was 3.4%, dual traditional Chinese medicine extract saponin content were 3.52%.