| Objective:To study ghrelin effect on the motor function of the rat gastrointestinal tract.Methods:Prepare postoperative bowel in the rat model with dynamic obstacles. To watch the effects of intravenous injection of ghrelin, motilin or CGRP on gastric emptying and small intestine transmission function after gavage administration with99mTc-methyl cellulose.Results:After injection of FG into the HIP or PVN, retrogradely labeled neurons and neurofibra were found in the the HIP or PVN, immunohistochemical staining sections of ghrelin were found two types of labeled neurons in the HIP or PVN, which retrolabeled neurons with FG or CY3immunopositive. The amplitude of gastric motility in conscious rats was dose-dependently increased by micro injection of ghrelin into HIP, and the effects were reduced by Ghrelin receptor antagonist D-Lys3-GHRP-6.Conclusion:Ghrelin appears, therefore, as a strong gastrokinetic agent. It could dramatically accelerate the normal emptying process in conscious unoperated rats, and it was the most potent drug to reverse postoperative gastric ileus. Ghrelin could also accelerate the transit of the small intestine but had no effect on the colon. |
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