Obese Mice Adipose Tissue RIP140 Gene Expression Induced By High-fat Diet C57BL / 6J

Objective:To study the expression level of RIP140mRNA in adipose tissue of high fat diet-induced obese mice and the correlation with insulin resistance.Methods:Male C57BL/6J mice were divided into two groups:normal fat diet group (NFD) and high fat diet group (HFD), fed respectively for14weeks. Mice in HFD group whose body weight were20%higher than average weight of NFD mice were selected as obese mice. The triglycerides(TG),total cholesterol(TC),fast blood glucose(FBG), fast insulin (FIns) levels of the NFD group and obese mice were assayed and the HOMA-IR were calculated. The expression of RIP140mRNA in epididymal adipose tissue was examined by RT-PCR.Results:A total of12mice in HFD group were recruited into the obesity group. The level of TG, TC, FBG, FIns(P<0.05,HOMA-IR(P<0.01)in obesity group were higher than those of control group; The expression of RIP140mRNA in adipose increased significantly in obesity group(P<0.05) compared with the controls. The correlation analysis showed that the expression of RIP140mRNA in mice adipose were positively correlated with HOMA-IR(r=0.465, P<0.05)and TG (r=0.526, P<0.05), but not with TC, FBG and FIns.Conclusion:The expression of RIP140mRNA in adipose tissue is increased and positively correlated with insulin resistance in mice fed with high fat diet.