Establishment And Arsenic Trioxide MDR Of Gastric Cancer Cell Line SGC7901 / ADM's Role And Reversal Drug

Objective:To establish the multidrug-resistant cell models of gastric cancer cell line SGC7901/ADM in vitro, detecting its biological characteristics, observating its multi-drug resistance mechanisms, study the MDR reversing effect treated with arsenic trioxide (AS2O3).Methods:Multidrug-resistance human gastric carcinoma cell line (SGC7901/ADM) was established in vitro by gradually increased the density of adriamycin (ADM).The effect of AS2O3on SGC7901/ADM was treated, MTT assay was used to determine the sensitivity of multidrug-resistant cell to anticarcinogens, Immunocytochemistry (PV-method) was used to detect the expressions of P-gp,Caspase3,and flow cytometry (FCM) was applied to check apoptosis rate in tumor cells.Results:The resistance of SGC7901/ADM cells to ADM、VCR and TAX were7.49、7.56and5.33times, respectively. After48h precondition with0.05mmol/L AS2O3, resistance index (RI) of SGC7901/ADM decreased significantly (P<0.05).Reversing effect of P-gp and Caspase3expressions were found in SGC7901/ADM cell line when treated with As2O3,and cell apoptosis were induced, augmented with the increasing concentration of AS2O3(P＜0.01).Conclusions:SGC7901/ADM could be established from SGC7901/S cell lines by gradually increased the density of adriamycin (ADM). Multi-drug resistance mechanisms of SGC7901/ADM is related to MDR1protein, AS2O3can reverse drug resistance of SGC7901/ADM cells by the way of dose-dependent.