Identification And Quality Of Authenticity Black Fruit Wolfberry

Solanaceae black fruit wolfberry (Lycium rwthenicum Murray)ripe fruit plays a very important role in China’s traditional ethnic medicine. According to Tibetan medical classic"crystal beads Materia Medica "records in detail:black wolfberry fruit sweet natured, clearing heat,the treatment of heart fever,heart disease,irregular menstruation, menopause and other diseases have a very significant effect.Black fruit wolfberry fruits rich in anthocyanins Natural Pigment,betaine and other ingredients,modern pharmacology proof black fruit wolfberry physiologically active components related with the class.In recent years,the efficacy of black and white thorn wolfberry fruit were received widespread attention due to the shape of black fruit and white thorn Lycium berry is very similar to the two single is difficult to distinguish with the naked eye, and now the white thorn in Chinese herbal medicine market black fruit wolfberry main adulterants.Black fruit wolfberry can improve the body’s immune system, anti-aging;results show that long-term use of wild black fruit wolfberry also has a vision to protect young people,a significant role in pseudomyopia, vertigo and dizziness in the elderly, cataracts and dry eye symptoms and have a good health care.Meanwhile,black fruit wolfberry can also prevent diabetes,and has significant anti-fatigue,protect the liver,anti-tumor,clear the blood vessels,lower blood pressure,protect the cardiovascular system and other effects.At present,although the emergence of black fruit wolfberry fruit efficacy reported,but has yet to see the black fruit wolfberry reported studies on the active ingredient.Black fruit wolfberry fruits rich in anthocyanins Natural Pigment, betaine and other ingredients,such supposedly physiologically active ingredient with black fruit wolfberry related.Zygophyllaceae(Zygophyllaceae)plant white thorn(Nitraria tangutorum Bobr)belongs Nitraria(Nitraria),also known as acid fat, Mongolian Chen Hal yellow box.Western minority Uighur,Tibetan,Mongolian and many other minorities in medicine in the form of a large compound that appears.White thorn fruit s rich n amino acids,vitamins{111},flavonoids,saponins,alkaloids,{112},minerals and other active ingredients and nutrition.White thorn fruit as recorded in the"Chinese desert Medicinal Plants"in a traditional ethnic herbs,fruit used to treat colds,lack of breast milk,blood deficiency,indigestion,spleen and stomach.White thorn leaves then for irregular menstruation,arrhythmia,angina has a good effect.In recent years,the efficacy of black and white thorn wolfberry fruit were received widespread attention due to both fruit shape is very similar to the naked eye is difficult to distinguish,are herbal white thorn for the black market in major Chinese wolfberry fruit adulterants.The experiment by means of molecular identification of learning to distinguish,then phylogenetic tree based on ITS sequences of black wolfberry,analyze quality differences based on different groups of black wolfberry Lycium black sequence formation.Then through the black fruit anthocyanins and betaine Lycium Lycium created for black draft quality control standards provide,and with the white and red thorn Lycium comparison.The experiment by means of molecular identification of learning to distinguish,then phylogenetic tree based on ITS sequences of black wolfberry,analyze quality differences based on different groups of black wolfberry Lycium black sequence formation.Then through the black fruit anthocyanins wolfberry and Betaine establish quality control standards for the black fruit wolfberry provide experimental basis,and with the white and red thorn Lycium comparison.In this paper,the main producing areas of the country(Xinjiang Kashgar,Yarkand county,Altay,.Jinghe County,Qinghai Golmud,Nuomuhong other regions)of11batches of black fruit wolfberry done authenticity of identification,and all batches of black fruit Lycium anthocyanin ingredients do the research,the following are the main contents of this experiment:Chapter oneldentification of learning methods using molecular phylogenetic tree based on ITS sequences of black fruit wolfberry,and then analyze the quality difference black wolfberry Lycium sequence,depending on the formation of black groups,and identify the different origin of black fruit wolfberry authenticity from the perspective of molecular biology.Chapter III using RP-HPLC on a black-and-white thorn in wolfberry fruit anthocyanins content were measured to determine the anthocyanin composition of the RP-HPLC best black and white thorn Lycium extraction process,and the extraction conditions were optimized.In this study,13peaks were detected by scanning the spectrum are the maximum absorption wavelength525nm, initially identified as anthocyanins ingredients. Chromatographic conditions C18ODS80Ts QA(250×4.9mm,7um id,Tosoh,Tokyo,Japan),the guard column:Kromasil C18column(12×6.6mm,5um).Mobile phase:mobile phase:formicAcid solution (0.3%:12%;B:trifluoroacetic acid)in acetonitrile(18%in methanol); gradient t:0-5min-l-min-7-min(0%A-0%a-37%A-53%A);detection wavelength:525nm;flow rate was0.8ml/min;injection volume was25ul;DAD detector scanning wavelength200-800nm.30℃;injection volume injection volume:20μL.2The use of mass spectrometry for identification of isolated anthocyanin intakeBy LC-MS LC MS system will first batch of black fruit wolfberry measuring samples/MSD ion trap data analysis software to calculate molecular weight,and the measurement sample separation.Preliminary identification of the black wolfberry fruit of13anthocyanins.Chapter IIIHPLC-ELSD using the contents of the black and ordinary red wolfberry wolfberry fruit betaine component to determine chromatographic conditions were optimized in order to determine the optimum chromatographic conditions,and to analyze the differences and common red black fruit wolfberry Lycium between betaine.Upon examination,no significant differences black fruit wolfberry Lycium both ordinary red betaine content.Chromatographic conditions Hypersil NH2column:(230mm x4.2mm,5μm);mobile phase:and tetrahydrofuran-methanol-3%trifluoroacetic acid(61:30:9),the flow rate was10mL/min;drift.tube temperature is91℃;column temperature is22℃;injection volume:10μ.