Technology And Efficacy Of Liver Hangover Hovenia Square Optimization

[Objective]Screening of Hovenia dulcis Thunb Jiejiu recipe drug ratio; to optimize extraction process; A preliminary study of Hovenia dulcis Thunb Jiejiu recipe’s hepatoprotective efficacy.[Methods]1.Screening of Hovenia dulcis Thunb Jiejiu recipe drug ratio:(1) Set Hovenia dulcis Thunb, hawthorn, acanthopanax senticosus as3factors, each factor have3levels. By using the orthogonal design, Water extraction, concentrated it to a suitable concentration;(2) Copy the acute alcoholic live injury in mice model, take ALT, AST of serum as indicators, reference the liver index, observe the pathological changes of liver;(3) Using the comprehensive evaluation method to evaluate different drug ratio’s efficacy of orthogonal design and determine the best proportion of drugs.2.Optimization of extraction process of Hovenia dulcis Thunb Jiejiu recipe:(1)With dihydromyricetin and oleanolic acid as the reference substance, establish a detection method to the total flavonoids and total saponins of Hovenia dulcis Thunb Jiejiu recipe.Take total flavonoids, total saponins as index, with single factor investigation to extraction times, extraction solvent, grinding degree, temperature.(2) With dihydromyricetin and oleanolic acid as the reference substance, by using the orthogonal experimental design, observe the4factors:ethanol concentration, Material liquid ratio, degree of grinding and extraction time, to optimize the extraction process. 3.A preliminary study on the Hepatoprotective effect of Hovenia dulcis Thunb Jiejiu recipe:Copy the acute alcoholic liver injury in mice model, observe the low, medium and high dose of Hovenia dulcis thunb jiejiu recipe’s influence to serum ALT, AST, CHO and the liver cofficience.[Results]1.The best drug ratio of Hovenia dulcis Thunb Jiejiu recipe is Hovenia dulcis Thunb:Hawthorn:Acanthopanax senticosus (13.5g,7.5g,10.5g).2.The optimum extraction process of semen Hoveniae Jiejiu recipe is10mesh crude medicine,50%ethanol, soak for an hour, extracting for2times, material liquid ratio(1:10、1:8), extracting time (2.5hours、2.0hours).3. Hovenia dulcis Thunb Jiejiu recipe can significantly decrease the serum ALT, AST, CHO content, have good efficacy of solution wine and protect liver, the medium dose is relatively the best in show.[Conclusion]The best drug ratio of Hovenia dulcis Thunb jiejiu recipe is determined. Extraction technology is reasonable and feasible, Hovenia dulcis thunb jiejiu recipe has good effivavy of solution wine and protect liver.