Experimental Study Of A Diabetic Ulcer Healing Effect Of Skin Cream Promoted

Purpose： Establishing diabetic skin ulcer model in rats，using Yixiao ointment treatment，observing the effect of Yixiao ointment on promoting wound healing，and discussing the mainmechanism，through which we can provide reasonable scientific basis for Yixiao ointment inclinical treatment of diabetic ulcer．Material and Method：Design：a randomized controlled animal experiment Materials：Clean level of male rats：112，body weight30g-40g；Yixiao ointment and Kangfuxin LiquidPaste were both provided by the Liaoning University of Traditional Chinese MedicineAffiliated Hospital Pharmacy；rat immunohisto-chemical antibody was provided by BeijingBoosen Biological Technology Company；SP and DAB kits were provided by Wuhan bosterBiological Engineering Company．Methods：84models were randomly divided into threegroups, respectively as the experimental group positive control group and model controlgroup，with28rats in each group；another28were taken as normal control group：theexperimental group were used Yixiao ointment，the positive control group were sprayedKangfuxin Liquid and the model control group were with physiology saline water and steriledressing．Regular macroscopic observation ulcer area size，surface secretions，amount ofexudate and granulation conditions．After modeling (one week，two weeks，three weeks andfour weeks)，we measured the area of back wound and calculated the area and healing rate ofwound；at the same time, we made local wound muscle tissue sections to do，respectively，routine pathology all examination and immunohisto chemical examination of plateletendothelial cell adhesion molecule (Cluster of Differentiation31，CD31)，vascularendothelial growth factor (Vascular Endothelial Growth Factor，VEGF) and hypoxiainducible factor-1(Hypoxia Inducible Factor-1，HIF-1)．Results：All rats did not die unexpectedly at the end of the experiment．After modeling (oneweek，two weeks，three weeks and four weeks)，the experimental group wound area andcontraction rate were better than those of the positive control group and model controlgroup(P＜0.05)；after modeling (one week，two weeks，three weeks and four weeks)，positive expressions of immunohisto chemical CD31，VEGF and HIF-1in the experimental group andpositive control group were better than that in the model control group，and it was better in theexperimental group than that in the positive control group (P＜0.01)．Conclusion：Positive effect of Yixiao ointment treatment of diabetic skin ulcer issignificant．Through promoting CD31，VEGF and HIF-1gene expression，it can promotewound capillary，improve blood supply of skin ulcer of diabetic rats，promote the formation ofgranulation tissues，accelerate the ulcer wound repair and improve the healing rate of ulcerskin of diabetic rats．The treatment effect of Yixiao ointment is much better than that ofKangfuxin solution．...