Picrorhiza Particles Neuroprotective Effect In A Rat Model Of Optic Nerve Crush

Back ground: The optic nerve is an important structure of conducting visual preferences, many eye diseases such as glaucoma, traumatic optic nerve injury and ischemic optic neuropathy is likely to lead to optic nerve injury causing visual function decline, the only treatment for these diseases have not effectively, hu coptis chinensis and its effective components have been applied to the brain nerve damage, has certain protective effect on the survival of the neurons in the brain, rhizoma coptidis hu whether can be applied to patients with optic nerve injury remains to be further research.Purpose:Study traditional Chinese medicine rhizoma coptidis hu granules on rats with optic nerve clamp is optic nerve protection task model, to exp lore its action mechanism, and provide reference for clinical prevention an d treatment of eye disease related.Material and method:1、Main reagents and instruments:picrorhizae particles (Jiangyin, Jiangsu Province Pharmaceutical Co., Ltd.);10%chloral hydrate (Sinopharm Chemical Reagent Co., Ltd.); Weihua(?) povidone iodine (Liaoning Weihua Pharmaceutical Co., Ltd.; China People Ministry of health of the Republic Wei Zi (2006) No.0009); Benoxil(?) oxybuprocaine hydrochloride eye drops (Santen pharmaceutical Co., Ltd.; repackaging approval number Zhunzi:J20070072); erythromycin ointment (Baijingyu Nanjing pharmaceutical Co., Ltd.; Zhunzi:H32025968). Stereo microscope (Leica United States); micro tweezers1; microscopic geniculate tweezers1; micro scissors1; special micro tweezers1; micro needle holder1;2ml syringe certain;10-0several silk, medical cotton swab several.10%chloral hydrate (Sinopharm Chemical Reagent Co., Ltd.; batch number20110509); America Chardonnay(?) compound tropicamide eye drops (Santen Pharmaceutical Co., Ltd. repackaging Sinopharm quasi-approval number:J20110007); toluidine blue reagent (Amresco,0672), an anti-from Beijing Biosynthesis Biotechnology Co., Limited. SP kit, DAB chromogenic kit from Beijing Biosynthesis Biotechnology Co., Limited. Neutral gum and hematoxylin dye forms by the Liaoning University of Traditional Chinese Medicine Laboratory.2、Methods:Will be32adult male Wistar rats by weight stratified randomly divided into normal control group, simple optic nerve crush group, simple picrorhizae gavage group, optic nerve crush combined picrorhizae gavage group each group of eight only. In rats at2mm ball with a certain intensity of the reverse grip tweezers clamp5seconds perpendicular to the optic nerve, Optic nerve pliers task model is established, simple picrorhizae gavage group and combined optic nerve of rats were fed picrorhizae respectively1days prior to the building, building on the same day, postoperative1day,3day,5days,9days,26days and28days, rats were given according to the amount of10mg/100g particles picrorhizae aqueous gavage, after injury After28days the animals were sacrificed to the eye, the optic nerve specimens, frozen sections. Were used hematoxylin-eosin (HE) staining, toluidine blue staining and glial fibrillary acidic protein (GFAP) immunohistochemical staining method, the optic nerve tissue sections stained in each group, morphological comparison between groups, and retinal thickness, ganglion cells, glial cells and GFAP positive cells were analyzed statistically.Results:1、Compared with normal control group, the optic nerve can cause thinning of the retina thickness (P<0.05), retinal ganglion cell loss and at the same time damage cavity obviously reduced in the optic nerve in rats, GFAP positive astrocytes proliferation response is obviously, cells swelled coarsens, shorter, structure disorder;2. Body weight administered alone picrorhizae10mg/100g rats retina and optic nerve structure and the normal control group had no significant difference (P>0.05);Tip10mg/100g dose picrorhizae particles on rat retina and optic nerve CRUSH toxic side effects. 3. Injury Joint picrorhizae10mg/100g weight administration can inhibit optic nerve crush induced changes in retinal thickness (P<0.05) as well as loss of optic nerve axons, While reducing the optic nerve glial cell proliferation responses (P<0.05); Tip this dose of particles can be partially restored picrorhizae rat optic nerve injury caused by structural changes in the retina and optic nerve.Conclusion:1、picrorhizae particles on rat optic nerve CRUSH model has a protective effect.2、picrorhizae particles neuroprotective effect by inhibiting microglial activation.